Evaluation of Mutations in 23S rRNA, rdxA and frxA Genes of Helicobacter pylori in Paraffin-Embedded Gastric Biopsy Specimens from Iranian Gastric Cancer and Gastritis Patients.

Journal of Gastrointestinal Cancer
Samad BeheshtirouyVahideh Tarhriz

Abstract

Helicobacter pylori (H. pylori) infection is considered as one of the main cause of gastric cancer. Treatment failure of the infection often occurs due to antibiotic resistance. Herein, we aimed to evaluate the mutations in 23S rRNA gene of H. pylori which are associated with clarithromycin resistance and in rdxA and frxA genes of the bacterium which may be associated with metronidazole resistance, in paraffin-embedded gastric biopsies from patients with gastric adenocarcinoma and gastritis in Tabriz, the northwest of Iran. In the study, 80 paraffin-embedded tissue sections from 40 gastric cancer and 40 gastritis patients in the Imam Reza hospital, Tabriz, Iran were collected. The existence of ureC gene was verified by PCR method. Genotypical clarithromycin resistance was investigated by real-time PCR method and determination of the melting temperature. PCR reaction and sequencing were used for the evaluation of mutations in rdxA and frxA genes. The results of ureC amplification showed that DNA of H. pylori was present in the 82.66% of the obtained DNA samples. About 45.16% of samples were resistant to the clarithromycin and 53.22% of them were resistant to the metronidazole. Based on the results from real-time PCR, the frequen...Continue Reading

References

Oct 31, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V Herrera, J Parsonnet
Nov 9, 2011·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Amin T AbadiAshraf M Mobarez
Dec 18, 2013·Journal of Medical Microbiology·Vincenzo De FrancescoDino Vaira
Mar 13, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Parisa KarimiFarin Kamangar
Apr 24, 2014·Journal of Medical Microbiology·Hanafiah AlfizahMohamed Ramelah
Apr 25, 2014·Advanced Biomedical Research·Nasrin MirzaeiHajieh Ghasemian Safaei
Dec 24, 2015·Alimentary Pharmacology & Therapeutics·I ThungM A Valasek
Oct 28, 2017·The Journal of Antimicrobial Chemotherapy·Simon A Dingsdag, Neil Hunter
Nov 17, 2017·European Journal of Clinical Investigation·Rocco Maurizio ZagariFranco Bazzoli
Feb 7, 2018·Frontiers in Microbiology·Paula DíazAndrew F G Quest
Feb 16, 2018·Cancer Management and Research·Robert SitarzWojciech P Polkowski

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Jul 21, 2021·BMC Microbiology·Sara EbrahimzadehShirin Eyvazi

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