Evaluation of Mycotoxin Screening Tests in a Verification Study Involving First Time Users

Toxins
Veronica M T LattanzioMichelangelo Pascale

Abstract

(AFB₁) in maize and wheat using LFD and LC-HRMS, respectively. The results of analyses were used to calculate intermediate precision (RSDip, covering the inter-analyst variability in preparing the analytical samples and the precision under repeatability conditions) cut-off values and false suspect rates. RSDip ranged from 6.5% to 30% for DON, and from 16% to 33% for AFB₁. The highest obtained variances were associated with the AFB₁ analyses due to working with much lower mass fractions. The rate of false suspect results were lower than 0.1% for all tested methods. All methods showed a fit-for-purpose method performance profile, which allowed a clear distinction of samples containing the analytes at the screening target concentration (STC) from negative control samples. Moreover, the first time users obtained method performances similar to those obtained for validation studies previously performed on the screening methods included in the training course.

References

Jul 1, 1988·Biomedical & Environmental Mass Spectrometry·C J ChandlerJ A Edgar
Jan 6, 2006·Food Additives and Contaminants·T B Whitaker
Jul 1, 2010·Prikladnaia biokhimiia i mikrobiologiia·A E UrusovB B Dzantiev
May 1, 2012·Analytical and Bioanalytical Chemistry·Laura AnfossiGianfranco Giraudi
Apr 9, 2013·Mycotoxin Research·Anne-Marie TurcotteBrett Tague
Jul 28, 2016·Journal of AOAC International·Gordon S Shephard

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