Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60.

Helicobacter
Varsha SinghGopal Nath

Abstract

To comparatively evaluate a new nested set of primers designed for the detection of Helicobacter pylori targeting a highly conserved heat shock protein gene (Hsp60). A total of 60 subjects having peptic ulcer diseases were tested for the detection of H. pylori using rapid urease test (RUT), histology, culture, and polymerase chain reaction (PCR) in their antral biopsy specimens. A newly designed Hsp60 gene-based primer set was evaluated against commonly used PCR primers for detection of H. pylori. Forty-six of the 60 study subjects were found positive for culture isolation and all the 46 culture-positive specimens were also positive with Hsp60 gene PCR. Of the 46 culture-positive specimens, 44 were positive for 16S rRNA gene, ureC gene, RUT, and histology whereas only 29 were positive with ureA gene PCR. Of the 14 culture-negative subjects, 10 were positive with 16S rRNA gene, 4 were positive with ureC (glmM) gene PCR, and 2 were positive with RUT and 1 was positive on histology. This study shows that nested amplification targeting Hsp60 gene is the most sensitive and specific with LR+ and LR- values of proportional, variant and 0, respectively, when compared with the other three PCR methods. Also, HSP60 gene-specific nested pr...Continue Reading

References

Jan 1, 1992·Journal of Clinical Microbiology·M HammarP W O'Toole
Jan 1, 1992·Journal of Clinical Microbiology·C L ClaytonS Tabaqchali
Apr 1, 1991·Journal of Clinical Microbiology·J L ValentineJ D Dick
Mar 1, 1991·Journal of Gastroenterology and Hepatology·D Y Graham
Jun 1, 1991·Journal of Clinical Pathology·C ClaytonS Tabaqchali
Feb 1, 1994·Journal of Clinical Microbiology·S FujimotoM J Blaser
Nov 1, 1996·Journal of Clinical Microbiology·S K ChongC H Lee
Jan 25, 1997·Lancet·C S GoodwinT C Northfield
Oct 23, 1997·Clinical Microbiology Reviews·B E DunnM J Blaser
Feb 13, 1999·Journal of Clinical Microbiology·J J LuC H Lee
Mar 11, 2000·Digestive Diseases and Sciences·S S Khanolkar-GaitondeC T Stadtländer

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Citations

Jan 9, 2009·Journal of Clinical Microbiology·Mitsushige SugimotoDavid Y Graham
Mar 8, 2013·World Journal of Gastroenterology : WJG·Saurabh Kumar PatelGopal Nath
Jul 24, 2014·BioMed Research International·Saurabh Kumar PatelGopal Nath
Sep 23, 2014·The Journal of Molecular Diagnostics : JMD·Margaret L Gulley, Douglas R Morgan
Aug 21, 2009·Digestive Diseases and Sciences·Tesiê L FonsecaPedro E A Silva
Sep 24, 2015·Digestive Diseases and Sciences·Nathan S S Atkinson, Barbara Braden
Jan 10, 2016·International Journal of Pediatric Otorhinolaryngology·Eleazar E Graterón CedeñoMaría Eugenia Cavazza Porro
Sep 6, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shrutkirti MishraGopal Nath
Sep 12, 2008·Helicobacter·Marta GranstromFrancis Mégraud
Apr 21, 2016·Polish Journal of Veterinary Sciences·M JankowskiM Biernat
Jul 1, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Reza KhasheiMaryam Sohrabi
Sep 30, 2017·Helicobacter·Fatemeh KhadangiMohammad Amin Kerachian
Oct 4, 2014·World Journal of Gastroenterology : WJG·Saurabh Kumar PatelGopal Nath
Apr 24, 2018·Middle East Journal of Digestive Diseases·Seyed Masih FatemiHamid Tavakkoli
May 2, 2019·World Journal of Gastrointestinal Oncology·Ana Flávia Teixeira RossiAna Elizabete Silva

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