PMID: 9535544Apr 16, 1998Paper

Evaluation of neutrophil functions after experimental abdominal surgical trauma

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
H ShijoN Sakakibara

Abstract

In order to determine the effect of surgical trauma on neutrophil functions, we set up an experimental abdominal surgical model using rats and analyzed neutrophil functions. In addition, we measured tumor necrosis factor-alpha (TNF-alpha), cytokine-induced neutrophil chemoattractant/growth-regulated oncogene (CINC/GRO) and nitric oxide (NO) production. Male Sprague-Dawley rats, 8 weeks old and weighing 250-270 g, underwent laparotomy (4 rats for each experiment). After the operation, neutrophil chemotaxis was assayed using a modified Boyden chamber, and phagocytosis, active oxygen production and adhesion molecule expression were analyzed by flow cytometry. TNF-alpha and CINC/GRO levels were quantified by an immuno-dot-blot assay, and NO levels were measured by the Griess method. At the operation, NO inhibitor, N(G)-monomethyl-L-arginine acetate (L-NMMA, 40 mg) was intraperitoneally administered, and the effect of L-NMMA was studied. After the surgical trauma (24-48 h), blood neutrophil counts significantly increased (p < 0.001), and neutrophil chemotaxis, phagocytosis and active oxygen production were markedly enhanced (p < 0.01). Moreover, up-regulation of Mac-1 and down-regulation of L-selectin on neutrophils were observed (p...Continue Reading

Citations

Jun 3, 2004·Langenbeck's Archives of Surgery·Michael D Menger, Brigitte Vollmar
Dec 15, 2012·Surgical Endoscopy·Rodrigo RodriguesErmelindo Della Libera
Aug 9, 2002·Auris, Nasus, Larynx·Fuyuki EnomotoGinichirou Ichikawa
Nov 11, 2009·The Journal of Trauma·Stephanie A ValenteAaron J Clark
Apr 10, 2019·Immunological Investigations·Humera InayatAkhter Ali Baloch
Feb 26, 2011·Expert Review of Respiratory Medicine·Mario PerlAlfred Ayala
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S M DolgilevichC Eng
Sep 26, 2019·Frontiers in Immunology·Esmaeil MortazIan M Adcock
Jul 3, 2021·Journal of Clinical Medicine·Lukasz WozniakEwa Jablonska
Jul 13, 2021·Frontiers in Immunology·Suzanne H BongersNienke Vrisekoop

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