Evaluation of neutrophilic function (chemotaxis, phagocytosis and microbicidal activity) in healthy dogs and in dogs suffering from recurrent deep pyoderma

Veterinary Immunology and Immunopathology
P P Chammas, M K Hagiwara

Abstract

The modified Boyden's technique of chemotaxis ('leading front' method) and the bacterial killing test with Staphylococcus sp. stained with acridine orange were performed with suspensions of granulocytes from 50 healthy dogs. Lipopolisacharide of Escherichia coli, in normal dog serum was used as the chemotactic factor. The mean value for the chemotactic differential found was 30.41 +/- 12.14 mu. The optimal concentration of bacteria and opsonins (normal dog serum) was 4 and 15%, respectively, and the ideal incubation period was 30 min. The mean values obtained for phagocytosis percentage, number of bacteria per PMN, phagocytosis index and percentage of dead bacteria, were 97.01 +/- 3.22%, 22.20 +/- 4.6, 21.53 +/- 4.50 and 45.30 +/- 9.18%, respectively. Granulocyte functions in 18 dogs with recurrent, chronic, deep pyoderma were assessed by the same methods. No significant difference between the two groups, for any of the evaluated parameters, was found. For elucidation of the pathogenesis of recurrent pyoderma, further studies involving humoral and cellular immunity and the complement system are required.

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Citations

May 26, 2005·Veterinary Immunology and Immunopathology·Marcela López-HurtadoFernando M Guerra-Infante
Oct 14, 2008·The Journal of Trauma·Grzegorz SzczêsnyPiotr Wasilewski
Jan 15, 2004·American Journal of Veterinary Research·Douglas J WeissBruce Walcheck
Mar 20, 2004·Veterinary Dermatology·Aiden P Foster
Jan 29, 2021·Veterinary Medicine International·Andrea MarchegianiSusan Paterson

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