PMID: 9659114Jul 11, 1998Paper

Evaluation of new improved solution containing trehalose in free skin flap storage

British Journal of Plastic Surgery
A K KitaharaY Nishimura

Abstract

In this study, we evaluated a new intracellular type (IT-K) solution containing trehalose in a rabbit free skin flap storage model. Trehalose is a nonreducing disaccharide that can stabilise cell membranes under various stressful conditions. Seventy-two free skin flaps of the ear of rabbits were preserved in Euro-Collins (EC) solution or in IT-K solution for 24, 48, and 72 h at 4 degrees C. After completion of preservation, these flaps were replanted to the other ear by microsurgical techniques. Viability study and photo documentation were performed daily for 7 days. Tissue specimens were taken 24 h after vascular anastomosis, fixed in 10% formaldehyde and stained with haematoxylin and eosin (HE). Survival rates were analysed by Fisher's exact test for comparison of the two experimental groups. Values of P < 0.05 were considered to be statistically significant. After 7 days, a survival rate of 100% of flaps were observed in both solutions after 24 h of preservation. After preservation for 48 h in IT-K solution the survival rate was 100%. However, in EC solution survival decreased to 75% (9 of 12 preserved flaps survived). This difference increased to 33.3% (4 of 12 flaps) in EC solution and 91.6% (11 of 12 flaps) (P < 0.01) in ...Continue Reading

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Apr 1, 1996·The Journal of Surgical Research·A K KitaharaY Nishimura
Nov 1, 1996·Annals of Plastic Surgery·C W ZhanY Nishimura

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Citations

Sep 12, 2014·Current Genetics·Elis EleutherioRayne Magalhães

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