Evaluation of nitric oxide production by Leishmania infantum-infected dog macrophages

Immunopharmacology and Immunotoxicology
Mariaantonietta PanaroOlga Brandonisio

Abstract

Protozoa of the genus Leishmania (L.) infect reticuloendothelial cells of several mammalian species, including dogs, in which they often give rise to a chronic, not self-healing visceral disease. Since the parasitocidal mechanism of macrophages towards Leishmania in dog has not yet been well investigated, in this work we have evaluated in Leishmania infantum-infected macrophage cultures from 10 healthy dogs, killing capacity and nitric oxide (NO) production, in terms of nitrite (NO2) levels. Parallel experiments were performed on macrophages stimulated with both Concanavalin A (ConA)-activated PBMC supernatants and Salmonella typhimurium lipopolysaccharide (LPS), and in the same conditions, but in the presence of the NO synthase inhibitor L-N monomethylarginine (L-NMMA). In L. infantum-infected macrophages, nitric oxide production was observed at a concentration significantly higher after stimulation with both Con A-activated PBMC supernatants and LPS than that observed in uninfected cells cultured in medium alone, or infected cells unstimulated or stimulated by PBMC supernatants or LPS alone, respectively. Moreover, NO production was abolished in the presence of the NO synthase inhibitor L-NMMA. Finally, killing of Leishmania ...Continue Reading

References

Mar 1, 1992·Transactions of the Royal Society of Tropical Medicine and Hygiene·M GramicciaM Troiani
Mar 1, 1992·European Journal of Epidemiology·O BrandonisioV Puccini
May 1, 1981·The Journal of Experimental Medicine·H W Murray
Jun 1, 1995·Annals of Medicine·I Pörsti, I Paakkari
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Sep 1, 1996·Veterinary Immunology and Immunopathology·O BrandonisioV Puccini

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Citations

Jul 7, 2001·Comparative Immunology, Microbiology and Infectious Diseases·M A PanaroO Brandonisio
Sep 20, 2001·Comparative Immunology, Microbiology and Infectious Diseases·M SistoV Mitolo
Jan 12, 2010·Comparative Immunology, Microbiology and Infectious Diseases·T TrottaM A Panaro
Sep 22, 2018·Frontiers in Cellular and Infection Microbiology·Carla Maia, Lenea Campino

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