Evaluation of novel assays to assess the influence of different iron sources on the growth of Clostridium difficile

Anaerobe
Ramona C Cernat, Karen P Scott

Abstract

The ability of four Clostridium difficile strains to utilize various exogenous organic and inorganic iron sources for growth under iron-depleted (250 μM DPP) and iron-limited (75 μM DPP) conditions was analyzed in liquid broth cultures grown in tubes and in microtiter plates, and data compared with results from a bioassay developed on solid media. The growth profile of C. difficile varied depending on the iron source and availability. Addition of FeSO(4), FeCl(3), Fe citrate and ferritin allowed growth in an iron-depleted environment whereas glycoproteins (iron-saturated and low-iron lactoferrin, apo- and holo-transferrin) and heme proteins (hemoglobin, hematin and hemin) did not. All iron sources, except lactoferrin, were able to restore bacterial growth under iron-limited conditions to varying extents. The results demonstrated that the broth microtiter assay developed here was reproducible, reliable and convenient for high-throughput analysis of the growth of C. difficile compared to alternative traditional methods.

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Citations

Jan 14, 2016·The Journal of Antimicrobial Chemotherapy·C H ChiltonT M Monaghan
Jun 24, 2015·Journal of Cellular and Molecular Medicine·Wei WeiHao Zhang
Aug 6, 2013·FEMS Microbiology Ecology·Karen P ScottHarry J Flint
Oct 8, 2016·Frontiers in Microbiology·Pamela Vazquez-GutierrezChristophe Lacroix
Jul 31, 2013·Infection and Immunity·Claire JanoirBruno Dupuy
Nov 19, 2015·MBio·Alexandra DostalChristophe Chassard
Jan 9, 2019·Frontiers in Microbiology·Mareike BergesDieter Jahn
Jul 9, 2020·Immunology·Ana Sousa GerósJoe N Frost

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