Evaluation of novel urinary tract infection biomarkers in children

Pediatric Research
Joshua R WatsonAndrew L Schwaderer

Abstract

Pediatricians frequently use urinalysis to diagnose urinary tract infection (UTI) while awaiting urine culture results, but sensitivity and specificity of urinalysis are limited. This study evaluated the diagnostic accuracy of the antimicrobial peptides (AMPs) human α-defensin 5 (HD5) and human neutrophil peptides (HNP) 1-3 as novel UTI biomarkers in children. We prospectively enrolled 199 pediatric Emergency Department or Urgent Care patients evaluated for a UTI. Urine concentrations of HD5 and HNP1-3 were measured by enzyme-linked immunosorbent assay. Urine culture was the reference standard. Sensitivities and specificities of leukocyte esterase (LE), HD5, HNP1-3, and test combinations were compared. For predicting positive urine culture, the areas under the receiver-operating characteristic curves for HD5 and HNP1-3 were 0.86 (95% confidence interval (CI): 0.81-0.92) and 0.88 (95% CI: 0.82-0.93), respectively. Compared to LE ≥ trace, the combination test "LE and HD5" increased specificity by 6% (95% CI: 3-10%) without decreasing sensitivity. In the subgroup whose urine was collected by a clean-catch method, combination tests "LE and HD5" and "HD5 and HNP1-3" increased specificity by > 10% compared to LE alone. Urine AMP prof...Continue Reading

References

May 1, 1991·The Journal of Pediatrics·K N ShawJ S Schwartz
Dec 24, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T IhiS Matsukura
Feb 12, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·I TikhonovA Chyzh
Sep 14, 2000·Comprehensive Therapy·R S Dieter
Feb 16, 2005·The Journal of Urology·Andrew L Freedman, UNKNOWN Urologic Diseases in America Project
Dec 27, 2007·JAMA : the Journal of the American Medical Association·Nader ShaikhEllen R Wald
Mar 18, 2010·Anesthesiology·Patrick RayTim T Houle
Mar 26, 2010·The Lancet Infectious Diseases·Gabrielle J WilliamsJonathan C Craig
Aug 17, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·John David SpencerDavid S Hains
Apr 29, 2011·Kidney International·John D SpencerDavid S Hains
Oct 12, 2011·Pediatrics·Kenneth B Roberts, William V Raszka
Feb 24, 2012·PloS One·John David SpencerAndrew L Schwaderer
Jun 5, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·John David SpencerDavid S Hains
Jul 31, 2013·The American Journal of Emergency Medicine·Beena A KaziAndrea T Cruz

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Citations

Nov 24, 2019·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Nader ShaikhTimothy R Shope
Dec 29, 2018·Recent Patents on Inflammation & Allergy Drug Discovery·Alexander K C LeungKam L Hon
Apr 18, 2019·Urolithiasis·Kirsten KusumiAndrew Schwaderer
Jun 15, 2019·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Christina ChingBrian Becknell
Mar 25, 2019·The Journal of Pediatrics·Nader ShaikhTimothy R Shope
Jul 31, 2019·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·C BelleauM Bailhache
Jul 18, 2021·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Elaise B HillJohn D Spencer

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
urine collection

Software Mentioned

GraphPad Prism

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