Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate

Analytical and Bioanalytical Chemistry
Subash C B GopinathPenmetcha K R Kumar

Abstract

With a view to developing an economical and elegant biosensor chip, we compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficiency of reflection from gold layers of two different thicknesses was examined. The thicker gold layer (100 nm) on the single-crystal silicon showed a higher reflectivity than the thinner gold film (10 nm). The formation of a nucleic acid duplex and aptamer-ligand interactions were evaluated on these gold layers, and a crystalline silicon substrate coated with the 100-nm-thick gold layer is proposed as an alternative substrate for studies of interactions of biomolecules.

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Citations

Jun 19, 2012·The Analyst·Subash C B GopinathKiyomi Tsukagoshi
May 7, 2013·The Analyst·Marimuthu CitartanThean-Hock Tang
Mar 23, 2012·Sensors·Subash C B GopinathMakoto Fujimaki
Jul 17, 2015·BioMed Research International·Subash C B GopinathM K Md Arshad

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