Evaluation of oligosaccharide synthesis from lactose and lactulose using β-galactosidases from Kluyveromyces isolated from artisanal cheeses

Journal of Agricultural and Food Chemistry
Beatriz PadillaPaloma Manzanares

Abstract

The β-galactosidase activity of 15 Kluyveromyces strains isolated from cheese belonging to Kluyveromyces lactis and Kluyveromyces marxianus species was tested for the production of oligosaccharides derived from lactose (GOS) and lactulose (OsLu). All Kluyveromyces crude cell extracts (CEEs) produced GOS, such as 6-galactobiose and 3'-, 4'-, and 6'-galactosyl-lactose. At 4 h of reaction, the main trisaccharide formed was 6'-galactosyl-lactose (20 g/100 g of total carbohydrates). The formation of OsLu was also observed by all CEEs tested, with 6-galactobiose, 6'-galactosyl-lactulose, and 1-galactosyl-lactulose being found in all of the reaction mixtures. The synthesis of trisaccharides predominated over other oligosaccharides. K. marxianus strain O3 produced the highest yields of GOS and OsLu after 4 h of reaction, reaching 42 g/100 g of total carbohydrates (corresponding to 80% lactose hydrolysis) and 45 g/100 g of total carbohydrates (corresponding to 87% lactulose hydrolysis), respectively. Therefore, the present study contributes to a better insight into dairy Kluyveromyces β-galactosidases and shows the feasibility of these enzymes to transglycosylate lactose and lactulose, producing high yields of prebiotic oligosaccharides.

References

Feb 26, 2000·Enzyme and Microbial Technology·M A Boon van 't Riet K
Jun 1, 2000·Applied and Environmental Microbiology·H Kaplan, R W Hutkins
May 15, 2001·Regulatory Toxicology and Pharmacology : RTP·M W Pariza, E A Johnson
Nov 28, 2001·Journal of Applied Microbiology·C E RycroftR A Rastall
Aug 1, 1952·Archives of Biochemistry and Biophysics·M ARONSON
Jun 23, 2005·Critical Reviews in Food Science and Nutrition·Ana C AdamJulio Polaina
Jul 6, 2006·Journal of Agricultural and Food Chemistry·Barbara SplechtnaDietmar Haltrich
Dec 22, 2007·Journal of Agricultural and Food Chemistry·Cristina Martínez-VillaluengaMaria Luisa Jimeno
Apr 17, 2008·Journal of Agricultural and Food Chemistry·Alejandra Cardelle-CobasNieves Corzo
Apr 23, 2008·Applied Microbiology and Biotechnology·Gustavo Graciano FonsecaAndreas K Gombert
Apr 24, 2008·Bioprocess and Biosystems Engineering·Gurpreet KaurHarish Kumar
Apr 27, 2010·International Journal of Food Microbiology·Tine EbersbachTine Rask Licht
Mar 29, 2011·Journal of Biotechnology·Maria Rodriguez-FernandezJulio R Banga
Jun 28, 2011·International Journal of Food Microbiology·Alejandra Cardelle-CobasMarta Ávila
Dec 14, 2011·Journal of Agricultural and Food Chemistry·Ali OsmanDimitris Charalampopoulos
Feb 2, 2012·Journal of Agricultural and Food Chemistry·Alejandra Cardelle-CobasRobert A Rastall
Sep 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Daniel Obed Otieno

❮ Previous
Next ❯

Citations

Oct 15, 2013·World Journal of Microbiology & Biotechnology·Julia Marín-NavarroJulio Polaina
Apr 4, 2015·BioMed Research International·María FernándezClara G de los Reyes-Gavilán
Jul 14, 2017·Journal of the Science of Food and Agriculture·Rebaone LetsididiWanmeng Mu
Sep 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Sara C SilvérioLígia R Rodrigues
Jan 29, 2021·Journal of Food Science and Technology·Min SuChunhong Piao
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Rounak ChourasiaAmit Kumar Rai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.