Evaluation of opiate separation by high-resolution electrospray ionization-ion mobility spectrometry/mass spectrometry

Analytical Chemistry
Laura M Matz, Herbert H Hill

Abstract

The separation of opiates and the primary metabolites was evaluated with ESI-IMS/MS. Seven opiate molecules were analyzed, and spectra were shown for each compound. The IMS separation of two isomers (morphine and norcodeine) was shown with baseline separation. Differences in the mobilities were found for the nonacetylated, monoacetylated, and biacetylated compounds. In this study, two primary findings are reported. First, IMS can easily separate metabolic isomers, and second, the two-dimensional separation capability of IMS/MS can be employed to confidently identify and separate both the opiates and metabolites. Although previous IMS studies have shown the separation of isomers, this is the first example to show the capability of IMS to separate metabolic isomers (within 70 s), a significant advantage in high-throughput screening for pharmacokinetic studies. Second, the monoacetylated and biacetylated compounds were found to form more compact ions for the sodium adducts in comparison to the protonated molecular ions. On the basis of the mobilities, information on structures and conformation can be deduced when sodium and protonated ions are compared.

References

Feb 28, 1976·Journal of Chromatography·F W KarasekS H Kim
Aug 6, 1998·Journal of the American Society for Mass Spectrometry·A E CountermanD E Clemmer
Oct 30, 1998·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer
Sep 29, 1999·Analytical Chemistry·C A SrebalusD E Clemmer
Oct 28, 1999·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer

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Citations

Sep 8, 2010·Analytical and Bioanalytical Chemistry·Joanna RudnickaBogusław Buszewski
Mar 16, 2013·Analytical and Bioanalytical Chemistry·Michael D WilliamsHerbert H Hill
Mar 23, 2002·Journal of the American Society for Mass Spectrometry·Michael D LeavellDavid E Clemmer
Mar 4, 2008·Analytical Chemistry·Brian H ClowersRichard D Smith
Apr 11, 2012·International Journal of Molecular Sciences·N Elizabeth PryorChrista N Hestekin
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Aug 28, 2001·Journal of Mass Spectrometry : JMS
Jun 29, 2006·Journal of Psychosocial Oncology·Joan Framo RunfolaPatricia Sherman
Mar 9, 2013·Journal of Breath Research·Paweł MochalskiBogusław Buszewski
Aug 26, 2009·Rapid Communications in Mass Spectrometry : RCM·Jaakko LaakiaTapio Kotiaho
Jan 8, 2021·Rapid Communications in Mass Spectrometry : RCM·Younes Valadbeigi, Vahideh Ilbeigi
Feb 4, 2021·Journal of the American Society for Mass Spectrometry·Cameron N Naylor, Brian H Clowers
Mar 24, 2018·Talanta·Kendra J AdamsFrancisco Fernandez-Lima
Aug 30, 2003·Analytical Chemistry·T A BrettellR Saferstein
Apr 30, 2003·Journal of Mass Spectrometry : JMS·R KostiainenS Auriola

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