Abstract
Immunomagnetic selection of CD34(+) hematopoietic progenitor cells (HPC) using CliniMACS CD34 selection technology is widely used to provide high-purity HPC grafts. However, the number of nucleated cells and CD34+ cells recommended by the manufacturer for processing in a single procedure or with 1 vial of CD34 reagent is limited. In this retrospective evaluation of 643 CliniMACS CD34-selection procedures, we validated the capacity of CliniMACS tubing sets and CD34 reagent. Endpoints of this study were the recovery and purity of CD34+ cells, T-cell depletion efficiency and recovery of colony-forming units-granulocyte-macrophage (CFU-GM). Overloading normal or large-scale tubing sets with excess numbers of total nucleated cells, without exceeding the maximum number of CD34+ cells, had no significant effect on the recovery and purity of CD34+ cells. In contrast, overloading normal or large-scale tubing sets with excess numbers of CD34+ cells resulted in a significantly lower recovery of CD34+ cells. Furthermore, the separation capacity of 1 vial of CD34 reagent could be increased safely from 600 x 10(6) CD34+ cells to 1000 x 10(6) CD34+ cells with similar recovery of CD34(+) cells. Finally, T-cell depletion efficiency and the frac...Continue Reading
References
Jun 1, 1996·Journal of Hematotherapy·D R SutherlandI Chin-Yee
Feb 1, 1997·Journal of Hematotherapy·I McNieceS Miltenyi
Jun 19, 1998·Bone Marrow Transplantation·R HandgretingerT Klingebiel
Jun 1, 1999·Journal of Hematotherapy·M SchummR Handgretinger
Sep 12, 2000·Journal of Hematotherapy & Stem Cell Research·D DesprésH G Derigs
Apr 24, 2001·Arthritis and Rheumatism·R J VerburgJ M van Laar
Nov 24, 2001·Blood·V T Ho, R J Soiffer
Jul 9, 2002·British Journal of Haematology·Michael J WattsDavid C Linch
Sep 10, 2003·Transplantation Proceedings·D E R Sutherland
Jan 6, 2004·Bone Marrow Transplantation·P LangR Handgretinger
Dec 15, 2004·Journal of Clinical Apheresis·Fikret ArpaciBekir Oztürk
Dec 20, 2005·Journal of Neurology, Neurosurgery, and Psychiatry·J P A SamijnR Q Hintzen
Dec 24, 2005·British Journal of Haematology·Willem J van HeeckerenOmer N Koç
Jan 26, 2006·Stem Cells and Development·Claudia Del FanteLaura Salvaneschi
Jan 26, 2006·Stem Cells and Development·Paolo PerseghinAndrea Biondi
Citations
Nov 20, 2009·PloS One·Rogier M ThurlingsPaul P Tak
Apr 18, 2012·Cytotherapy·Maria Cristina ScerpaFrancesco Zinno
Nov 26, 2009·Cytotherapy·Francesco ZinnoGiancarlo Isacchi
Feb 9, 2010·Transfusion Medicine·F ZinnoG Isacchi
Aug 31, 2011·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Carolyn A Keever-TaylorRichard J O'Reilly
Aug 28, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Maaike G J M van BergenBert A van der Reijden