Evaluation of p16INK4a as a diagnostic tool in the triage of Pap smears demonstrating atypical squamous cells of undetermined significance

Cancer
Lisa DuncanElizabeth Hubbard

Abstract

P16(INK4a) (p16) has emerged as a biomarker for the detection of high-risk human papillomavirus (HR-HPV) in Papanicolaou (Pap) smears. Many studies have confirmed a strong correlation between p16 immunohistochemical positivity and high-grade squamous intraepithelial lesions (HSIL) of the cervix. Because p16 is predictive of HR-HPV and HSIL, it seems plausible that p16 could be used as a diagnostic tool to triage atypical squamous cells of undetermined significance (ASCUS) Pap smears. In this way, Pap smears with no p16 staining could be recategorized as negative for intraepithelial lesion or malignancy (NILM) before final case disposition, thus preventing unnecessary and costly follow-up. p16 immunostains were performed on 178 ThinPrep (Cytyc, Marlborough, Mass) Pap smears signed out as ASCUS among 5 cytopathologists. p16 stains were independently scored between 0 (no staining) and 4 (staining in cells with nuclear aberration) by either 2 or 3 pathologists. The p16 score was compared with both Hybrid Capture 2 (hc(2)) (Digene, Gaithersburg, Md) and follow-up (Pap smear and tissue) results. The sensitivity and specificity of p16 immunohistochemistry compared with both hc(2) and follow-up were not statistically significant, with ...Continue Reading

References

Feb 3, 1989·JAMA : the Journal of the American Medical Association·L G Koss
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M DürstH zur Hausen
Apr 1, 1995·American Journal of Epidemiology·M T FaheyP Macaskill
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S N KhleifP M Howley
Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·R KlaesM von Knebel Doeberitz
Apr 23, 2002·JAMA : the Journal of the American Medical Association·Diane SolomonUNKNOWN Bethesda 2001 Workshop
Apr 23, 2002·JAMA : the Journal of the American Medical Association·Thomas C WrightUNKNOWN ASCCP-Sponsored Consensus Conference
Jun 17, 2003·Journal of the National Cancer Institute. Monographs·Eduardo L Franco
Jul 23, 2003·Archives of Pathology & Laboratory Medicine·Mark Schiffman, Diane Solomon
Jul 23, 2003·Archives of Pathology & Laboratory Medicine·Attila T Lörincz, Ralph M Richart
Aug 19, 2003·Acta Cytologica·Chamsai PientongNuannit Ruckait
Oct 28, 2003·Cancer·Paul J J M KlinkhamerAntonius G J M Hanselaar
Jan 9, 2004·International Journal of Cancer. Journal International Du Cancer·Shaira SahebaliJohannes J Bogers
Feb 1, 1993·Trends in Cell Biology·K Helin, H Ed
Oct 14, 2004·Virchows Archiv : an International Journal of Pathology·Giovanni NegriEduard Egarter-Vigl
Dec 8, 2004·Acta Cytologica·Marcus J TrunkMagnus von Knebel Doeberitz
Aug 4, 2005·Diagnostic Cytopathology·Liron PantanowitzChristopher N Otis

❮ Previous
Next ❯

Citations

Jun 28, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Malgorzata LanowskaDorothee Speiser
Feb 4, 2009·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Michael H RohChristopher P Crum
Jan 22, 2011·Diagnostic Cytopathology·Francesc AlamedaSergi Serrano
Nov 26, 2009·Diagnostic Cytopathology·Trynda N ObergAmy C Clayton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.