PMID: 9542933May 23, 1998Paper

Evaluation of PCR for diagnosis of melioidosis

Journal of Clinical Microbiology
A HaaseBart J Currie

Abstract

Previously published PCR-based diagnostic tests for melioidosis were evaluated for clinical usefulness. A Burkholderia pseudomallei 16S rRNA-derived primer set had a sensitivity approaching 100% for clinical samples from 22 culture-confirmed cases of melioidosis and enabled diagnosis of 3 culture-negative cases. However, samples from 10 of 30 inpatients from Royal Darwin Hospital with other diagnoses were positive by PCR, giving a specificity of 67% and a positive predictive value of only 70%. Although there are a number of intriguing possible explanations for our results, concerns of inappropriate therapy resulting from a positive result by PCR have led us to forgo the advantage of rapid PCR diagnosis for melioidosis until a better system is validated.

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Citations

Jul 10, 2007·Journal of Travel Medicine·Henry Wilde, Chusana Suankratay
Apr 16, 2005·Clinical Microbiology Reviews·Allen C Cheng, Bart J Currie
Nov 13, 2004·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Herbert TomasoHeinrich Neubauer
Nov 5, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L D Sprague, H Neubauer
Jul 19, 2012·World Journal of Microbiology & Biotechnology·P TrivediH V Batra
May 1, 2020·Journal of Clinical Microbiology·Ornuma SangwichianGanjana Lertmemongkolchai
Feb 18, 2021·Future Microbiology·Ian GassiepPatrick N Harris
Apr 3, 2003·Journal of Feline Medicine and Surgery·C R O'BrienR Malik
Aug 8, 2006·Journal of Clinical Microbiology·Ella M MeumannBart J Currie
Oct 12, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M V JesudasonG Sridharan

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