Evaluation of performance and false positivity of Mediace RPR test that uses a chemistry autoanalyzer

The Korean journal of laboratory medicine
Jaekwang NohEun Young Song

Abstract

We evaluated the performance and false positive rate of Mediace RPR test (Sekisui, Japan), a newly introduced nontreponemal test using a chemistry autoanalyzer. The sensitivity of Mediace RPR test was analyzed using sera from 50 patients with syphilis in different stages (8 primary, 7 secondary, and 35 latent), 14 sera positive with fluorescent treponemal antibody absorption (FTA-ABS) IgM, and 74 sera positive with conventional rapid plasma regain (RPR) card test (Asan, Korea) and also positive with Treponema pallidum hemagglutination (TPHA) test or FTA-ABS IgG test. The specificity was analyzed on 108 healthy blood donors. We also performed RPR card test on 302 sera that had been tested positive with Mediace RPR test and also performed TPHA or FTA-ABS IgG test to analyze the false positive rate of Mediace RPR test. A cutoff value of 0.5 R.U. (RPR unit) was used for Mediace RPR test. Mediace RPR test on syphilitic sera of different stages (primary, secondary, and latent stages) and FTA-ABS IgM positive sera showed a sensitivity of 100%, 100%, 82.9% and 100%, respectively. Among the 74 sera positive with conventional RPR card test and TPHA or FTA-ABS IgG test, 55 were positive with Mediace test. The specificity of Mediace RPR te...Continue Reading

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Citations

May 11, 2010·European Journal of Medical Research·Xiaohui MoWeiming Gu
Sep 16, 2015·Journal of the European Academy of Dermatology and Venereology : JEADV·K MalmM Unemo
Apr 6, 2018·Journal of Clinical Microbiology·Alan M SanfilippoJohn L Schmitz
Sep 4, 2009·The Korean journal of laboratory medicine·Yeong Sic KimSoo-Young Kim

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