Evaluation of performance testing of different rapid diagnostic kits in comparison with EIAs to validate detection of hepatitis B virus among high risk group in Nigeria

Journal of Immunoassay & Immunochemistry
Abosede Yetunde AfolabiM O Adewumi

Abstract

Hepatitis B virus (HBV) causes chronic liver-associated diseases and its early detection is of high public health importance. Its diagnosis is mainly based on immunological assays among which Enzyme-Linked Immunosorbent Assay (ELISA) and rapid tests are the most common and widespread methods. However, a major challenge is the discordance of results of any two laboratory assays which cannot be easily resolved. Therefore, this study was designed to evaluate the validity and reliability of commercially available five rapid test kits in comparison with two Enzyme Immunoassays (EIAs) in Nigeria using hepatitis B surface antigen as a reference marker. A total of 100 sera of previously diagnosed consenting HBV-positive patients from private diagnostic laboratories in Ibadan between March and August, 2011 were tested using two EIA and five rapid commercially available HBV test kits in Nigeria. Data were analyzed by SPSS version 15, while bivariate and multivariate analyses were carried out to identify associations at P < 0.05 considered significant. Overall, the sensitivity rates of the two EIA kits were 100% and 99.9% (95% confidence interval [CI] = 98.9-99.7) with specificity of 100% and 99.9% (95% CI = 98.9-99.7), respectively. The ...Continue Reading

References

Aug 15, 2008·Journal of Clinical Microbiology·Yu-Huei LinHelen H Lee
Jan 22, 2011·World Journal of Gastroenterology : WJG·Jung Min Lee, Sang Hoon Ahn
Jul 23, 2013·Virology Journal·Wolfram H Gerlich
Jan 20, 2018·Journal of Virology & Antiviral Research·Macon D ColemanSung Ouk Kim

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Citations

Oct 8, 2020·Cells·Yinzong XiaoJessica Howell

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Methods Mentioned

BETA
ELISA

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