Evaluation of phenotypic and molecular methods for detection of oxacillin resistance in members of the Staphylococcus sciuri group

Journal of Clinical Microbiology
Srdjan StepanovicDonald Morrison

Abstract

In this paper we report on an experimental evaluation of phenotypic and molecular methods as means for the detection of oxacillin resistance in members of the Staphylococcus sciuri group. A total of 109 S. sciuri group member isolates (92 S. sciuri isolates, 9 S. lentus isolates, and 8 S. vitulinus isolates) were tested by the disk diffusion method, the agar dilution method, the oxacillin salt-agar screening method, slide latex agglutination for PBP 2a, and PCR assay for mecA as the reference method. The mecA gene was detected in 29 S. sciuri isolates, and the true-positive and true-negative results of the other tests were defined on the basis of the presence or the absence of the mecA gene. For the different methods evaluated, the sensitivities and specificities were as follows: for the disk diffusion test with a 1-microg oxacillin disk, 100% and 55.9%, respectively; for the disk diffusion test with a 30-mug cefoxitin disk, 93.5% and 100%, respectively; for the agar dilution method, 100% and 50%, respectively; for the oxacillin salt-agar screen test (with 6 microg of oxacillin per ml and 4% NaCl) 100% and 100%, respectively; and for the slide latex agglutination test for PBP 2a, 100% and 100%, respectively. The disk diffusion ...Continue Reading

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Citations

May 11, 2013·Veterinary Research Communications·Raghu Patil JunjappaSriram Padmanabhan
Aug 4, 2010·Antimicrobial Agents and Chemotherapy·Sae TsubakishitaKeiichi Hiramatsu
Sep 15, 2010·Antimicrobial Agents and Chemotherapy·J A SeverinUNKNOWN AMRIN Study Group
Jul 14, 2010·Ultramicroscopy·Rikke Louise MeyerFlemming Besenbacher
May 11, 2017·Annals of Clinical Microbiology and Antimicrobials·Kamelia OsmanMohamed Elhadidy
Jul 18, 2018·Polish Journal of Microbiology·Rocío A Ruiz-RomeroEstela T Méndez-Olvera

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