Evaluation of Plasma Adenosine as a Marker of Cardiovascular Risk: Analytical and Biological Considerations

Journal of the American Heart Association
Trevor SimardBenjamin Hibbert

Abstract

Background Adenosine is a ubiquitous regulatory molecule known to modulate signaling in many cells and processes vital to vascular homeostasis. While studies of adenosine receptors have dominated research in the field, quantification of adenosine systemically and locally remains limited owing largely to technical restrictions. Given the potential clinical implications of adenosine biology, there is a need for adequately powered studies examining the role of plasma adenosine in vascular health. We sought to describe the analytical and biological factors that affect quantification of adenosine in humans in a large, real-world cohort of patients undergoing evaluation for coronary artery disease. Methods and Results Between November 2016 and April 2018, we assessed 1141 patients undergoing angiography for evaluation of coronary artery disease. High-performance liquid chromatography was used for quantification of plasma adenosine concentration, yielding an analytical coefficient of variance (CVa) of 3.2%, intra-subject variance (CVi) 35.8% and inter-subject variance (CVg) 56.7%. Traditional cardiovascular risk factors, medications, and clinical presentation had no significant impact on adenosine levels. Conversely, increasing age (P...Continue Reading

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Citations

Dec 1, 2019·International Journal of Molecular Sciences·Emmanuel FenouilletJean Ruf
Jul 31, 2020·International Journal of Molecular Sciences·Marine GaudryFranck Paganelli
Sep 30, 2020·Cardiovascular Research·Franck PaganelliRégis Guieu
Nov 25, 2020·Stem Cells Translational Medicine·Adil RasheedCarolyn L Cummins
Oct 20, 2020·Circulation·Venkatesh L MurthyRavi V Shah
Dec 3, 2020·Clinical and Translational Science·Trevor SimardBenjamin Hibbert
Mar 3, 2020·Experimental and Molecular Pathology·Haochang HuShuangshuang Wang
Mar 25, 2021·Journal of Cardiovascular Pharmacology·Trevor SimardBenjamin Hibbert
Oct 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Barbara Kutryb-ZajacRyszard T Smolenski
Aug 28, 2021·Frontiers in Cardiovascular Medicine·Emilio Y Lucero García RojasRichard A Bond

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Methods Mentioned

BETA
coronary artery bypass
Assay

Software Mentioned

SAS
GraphPad Prism
Waters Empower
SigmaStat

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