Evaluation of poly{-N-isopropylacrylamide-co-[3-(methacryloylamino)propyl]trimethylammonium} as a stationary phase for capillary electrochromatography

Electrophoresis
Xin Zhang, Luis A Colón

Abstract

A cationic polyacrylamide-based stationary phase was synthesized and characterized for CEC. The stationary phase was prepared by radical copolymerization of N-isopropylacrylamide (NIPAAm) and (3-(methacryloylamino)propyl)trimethylammonium chloride (MAPTA), producing a copolymer attached to 5 microm porous silica particles. Fourier transform infrared spectroscopy and thermogravimetric analysis were used to characterize the copolymer. Under capillary electrochromatographic conditions, the poly-NIPAAm-co-MAPTA stationary phase showed to be stable in a wide pH range. The amino groups in the MAPTA provided an anodic EOF for CEC separation. The electroosmotic mobility changed less than 10% when the pH of the mobile phase was changed from 2 to 12. The run-to-run RSD of analyte migration time was less than 1.5% (n = 3), and the RSD of peak area was less than 3% (n = 3). The day-to-day RSD for migration time was less than 2% (n = 3). The polar groups present in the stationary phase contributed to the selectivity of the phase providing for hydrophilic interactions. In the separation of a series of neutral and acidic compounds, the stationary phase shows a mixed-mode separation mechanism with both hydrophobicity and hydrophilicity contrib...Continue Reading

References

Feb 10, 1998·Electrophoresis·L A ColónA M Fermier
Aug 29, 2000·Journal of Chromatography. a·F SvecJ M Fréchet
Feb 24, 2001·Electrophoresis·L A ColónR L Rodríguez
Jan 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hideko KanazawaTeruo Okano
Jul 31, 2002·The Analyst·José M Cintrón, Luis A Colón

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Citations

Jun 17, 2010·Se pu = Chinese journal of chromatography·Xucong LinZenghong Xie
Dec 1, 2006·Electrophoresis·Junjie OuHanfa Zou
May 15, 2009·Analytical Chemistry·Michael W L WatsonAaron R Wheeler

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