Evaluation of polycaprolactone scaffold with basic fibroblast growth factor and fibroblasts in an athymic rat model for anterior cruciate ligament reconstruction

Tissue Engineering. Part a
Natalie L LeongDavid R McAllister

Abstract

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury often necessitating surgery. Current surgical treatment options include ligament reconstruction with autograft or allograft, which have their inherent limitations. Thus, there is interest in a tissue-engineered substitute for use in ACL regeneration. However, there have been relatively few in vivo studies to date. In this study, an athymic rat model of ACL reconstruction was used to evaluate electrospun polycaprolactone (PCL) grafts, with and without the addition of basic fibroblast growth factor (bFGF) and human foreskin fibroblasts. We examined the regenerative potential of tissue-engineered ACL grafts using histology, immunohistochemistry, and mechanical testing up to 16 weeks postoperatively. Histology showed infiltration of the grafts with cells, and immunohistochemistry demonstrated aligned collagen deposition with minimal inflammatory reaction. Mechanical testing of the grafts demonstrated significantly higher mechanical properties than immediately postimplantation. Acellular grafts loaded with bFGF achieved 58.8% of the stiffness and 40.7% of the peak load of healthy native ACL. Grafts without bFGF achieved 31.3% of the stiffness and 28.2% of the pe...Continue Reading

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Citations

Apr 15, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Farideh MohammadianAbolfazl Akbarzadeh
Oct 27, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Natalie L LeongDavid R McAllister
Apr 13, 2017·Journal of Tissue Engineering and Regenerative Medicine·Kosei ShinoharaKyoko Ohno-Matsui
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May 20, 2019·BMC Musculoskeletal Disorders·Hirotaka MutsuzakiMasataka Sakane
Dec 12, 2019·Tissue Engineering and Regenerative Medicine·Wei Lee LimJia Xian Law
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Feb 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zifeng LinChang Du
Feb 9, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Cedryck VaquetteSaso Ivanovski
Dec 18, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Eduardo Henrique BackesLuiz Antonio Pessan

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Methods Mentioned

BETA
light microscopy
fluorescence microscopy
dissection

Software Mentioned

ImageJ

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