Evaluation of polymerase chain reaction amplification of Mycobacterium leprae-specific repetitive sequence in biopsy specimens from leprosy patients.

Journal of Clinical Microbiology
K H YoonJ D Kim

Abstract

Biopsy specimens were obtained from 102 leprosy patients before chemotherapy and examined by polymerase chain reaction (PCR) using the primers amplifying the 372-bp DNA of a repetitive sequence of Mycobacterium leprae. The PCR results were then compared with bacterial indices (BI) of slit-skin smears and biopsy specimens. The intensities of DNA bands were in general correlated with the numbers of acid-fast bacilli, and even a sample with only one organism gave a PCR positive result. Ten 5-micron sections from each frozen tissue sample were pooled and processed for DNA preparation. PCR was positive for 11 (73.3%) of 15 biopsy specimens with BI of 0 determined for the paraffin sections from the same biopsy samples. PCR also gave positive results for 84 (96.6%) of 87 BI positive biopsy samples. Although the difference in overall results between the two methods was not statistically significant, PCR seemed to have an advantage over microscopic examination in detecting M. leprae in biopsy specimens negative for acid-fast bacilli. Further evaluation of PCR using more specimens from leprosy patients who are bacteriologically negative is warranted to ensure PCR's advantage over the conventional microscopic examination for the diagnosis...Continue Reading

References

Nov 1, 1991·The American Review of Respiratory Disease·K D EisenachJ T Crawford
May 1, 1991·Journal of Clinical Microbiology·M Y de WitR A Hartskeerl
Oct 1, 1990·Molecular Microbiology·S A Woods, S T Cole
Jun 1, 1990·Journal of Clinical Microbiology·P W HermansJ D van Embden
Sep 1, 1990·Journal of Clinical Microbiology·B B PlikaytisT M Shinnick
Jul 1, 1989·Molecular Microbiology·A J HanceB Gicquel

❮ Previous
Next ❯

Citations

May 8, 2008·Archives of Dermatological Research·Isabela Maria Bernardes Goulart, Luiz Ricardo Goulart
Oct 12, 2011·Anais Brasileiros De Dermatologia·Andrezza Furquim da CruzAna Maria Ferreira Roselino
Nov 30, 2005·Muscle & Nerve·Francisco M Bezerra Da CunhaLineu C Werneck
May 29, 2007·Archives of Dermatological Research·Isabela Maria Bernardes GoulartLuiz Ricardo Goulart
May 24, 2005·FEMS Immunology and Medical Microbiology·Lucas Gomes PatrocínioRaul Negrão Fleury
Nov 13, 2015·Memórias do Instituto Oswaldo Cruz·Luana Nepomuceno Gondim Costa LimaLigia Kerr
Oct 30, 2016·International Journal of Rheumatic Diseases·Latika GuptaVikas Agarwal
Jan 3, 2006·Indian Journal of Pediatrics·R DayalM Natrajan
Sep 23, 2003·Current Opinion in Infectious Diseases·Andrew P Ustianowski, Diana N J Lockwood
Oct 21, 2006·FEMS Immunology and Medical Microbiology·Benjawan PhetsuksiriPatrick J Brennan
Apr 12, 2014·PLoS Neglected Tropical Diseases·Alejandra Nóbrega MartinezSinésio Talhari
Dec 10, 2019·Disease-a-month : DM·Sushma BommanavarKamran Habib Awan
Apr 9, 2004·Journal of Clinical Microbiology·Nathan A GroathouseVaralakshmi D Vissa
May 9, 1998·Journal of Clinical Microbiology·M KurabachewJ J Ryon
Apr 24, 2009·Journal of Clinical Microbiology·Miyako KimuraVaralakshmi Vissa
Jan 1, 1995·Journal of Clinical Microbiology·J WichitwechkarnS Peerapakorn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
N MisraI Nath
Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association
L ShiM Endoh
International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
A RafiJ L Stanford
International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
C K JobThomas P Gillis
© 2021 Meta ULC. All rights reserved