Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis

Journal of Feline Medicine and Surgery
Helen Kedward-DixonChristopher R Helps

Abstract

Feline infectious peritonitis (FIP) is a high mortality infectious disease. Single nucleotide polymorphisms (SNPs) in the genes encoding interferon gamma (IFNG), tumour necrosis factor alpha (TNFA) and dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN; CD209) have been associated with increased and decreased risk of developing FIP. This study was designed to determine whether these associations were present in a UK population of pedigree cats using samples from cats euthanased with a confirmed diagnosis (FIP, n = 22; non-FIP, n = 10) or clinically healthy cats over 11 years of age (n = 3). DNA was extracted from tissue (n = 32) or blood (n = 3) and PCR performed for regions of IFNG, TNFA and CD209. PCR amplicons were sequenced, each SNP genotype was determined, and genotype/allele frequency for each SNP and FIP status were compared. No significant association was found between the genotype and FIP status for any SNP analysed. There was a trend for the heterozygous CT genotype at both IFNG g.401 and IFNG g.408 to be associated with FIP (P = 0.13), but this genotype was also found in a substantial proportion of non-FIP cats. There was also a trend for the heterozygous CT genotype at IFNG g.428...Continue Reading

References

Apr 25, 2001·Journal of the American Veterinary Medical Association·B W RohrbachR B Wilson
Jul 5, 2005·Journal of Feline Medicine and Surgery·Loretta D Pesteanu-SomogyiBarrak M Pressler
Jun 17, 2006·Journal of Feline Medicine and Surgery·Maria E GelainSaverio Paltrinieri
Apr 15, 2008·The Veterinary Journal·Alessia Giordano, Saverio Paltrinieri
May 21, 2010·Journal of Virology·Andrew D ReganGary R Whittaker
Mar 9, 2012·Journal of Feline Medicine and Surgery·Kate A WorthingJacqueline M Norris
Feb 27, 2014·Veterinary Pathology·A Kipar, M L Meli

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.