Evaluation of polymorphisms in pbp4 gene and genetic diversity in penicillin-resistant, ampicillin-susceptible Enterococcus faecalis from hospitals in different states in Brazil

FEMS Microbiology Letters
Victor Hugo Pacagnelli InfanteAna Lucia da Costa Darini

Abstract

The aim of the present study was to verify whether penicillin-resistant, ampicillin-susceptible Enterococcus faecalis (PRASEF) occurred in Brazil prior to the beginning of the 21st century, and to verify whether ampicillin susceptibility can predict susceptibility to other β-lactams in E. faecalis with this inconsistent phenotype. The presence of polymorphisms in the pbp4 gene and genetic diversity among the isolates were investigated. Of 21 PRASEF analyzed, 5 (23.8%) and 4 (19.0%) were imipenem and piperacillin resistant simultaneously by disk diffusion and broth dilution respectively, contradicting the current internationally accepted standards of susceptibility testing. Sequencing of pbp4 gene revealed an amino acid substitution (Asp-573→Glu) in all PRASEF isolates but not in the penicillin-susceptible, ampicillin-susceptible E. faecalis. Most PRASEF (90.5%) had related pulsed-field gel electrophoresis profiles, but were different from other PRASEF described to date. Results demonstrate that penicillin-resistant, ampicillin-susceptible phenotype was already a reality in the 1990s in E. faecalis isolates in different Brazilian states, and some of these isolates were also imipenem- and piperacillin-resistant; therefore, intern...Continue Reading

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Citations

Jul 31, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Luciano Bernardino da CostaAdriana Gonçalves Oliveira
Jul 10, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Iwona GawryszewskaEwa Sadowy
Feb 1, 2019·Clinical Microbiology Reviews·Mónica García-Solache, Louis B Rice
Mar 3, 2020·Frontiers in Microbiology·Leonardo G Panunzi

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