Evaluation of post-thaw Asian elephant (Elephas maximus) spermatozoa using flow cytometry: the effects of extender and cryoprotectant

Theriogenology
Nikorn ThongtipAnuchai Pinyopummin

Abstract

Although the development of semen cryopreservation in the African elephants (Loxodonta africana) has been accomplished, effective procedures for cryopreservation of Asian elephant (Elephas maximus) spermatozoa have not been established. In the present study, we investigate the freezing methods for conservation of Asian elephant spermatozoa under field conditions and identify the most suitable freezing protocols which provide acceptable post-thaw semen quality. Semen was collected from two Asian elephant bulls (EM1 and EM2, 10 ejaculates from each bull) by manual manipulation and were assessed for volume, pH, sperm cell concentration, and progressive motility. Eight out of 20 ejaculates were of acceptable quality (progressive motility >/= 60%), and were used for cryopreservation studies. Semen were frozen in TEST + glycerol, TEST + DMSO, HEPT + glycerol, or HEPT + DMSO. The post-thaw progressive sperm motilities were assessed, and sperm cells were stained with PI and FITC-PNA for membrane and acrosomal integrity assessment using flow cytometry. Post-thaw progressive motility of spermatozoa (EM1: 42.0 +/- 4.3%; EM2: 26.0 +/- 17.3%) and the percentage of membrane and acrosome intact spermatozoa (EM1: 55.5 +/- 8.1%; EM2: 46.3 +/- 6...Continue Reading

References

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Citations

Sep 7, 2012·Reproduction, Fertility, and Development·P ImratT A E Stout
Mar 19, 2008·Reproductive Biology and Endocrinology : RB&E·Nikorn ThongtipAnuchai Pinyopummin
Jul 21, 2009·Reproductive Biology and Endocrinology : RB&E·Nikorn ThongtipKulnasan Saikhun
Mar 14, 2013·PloS One·Robert HermesThomas B Hildebrandt
Dec 9, 2008·Animal Reproduction Science·Joseph SaragustyThomas B Hildebrandt
Dec 30, 2008·Animal Reproduction Science·Joseph SaragustyRobert Hermes
Dec 6, 2007·Reproduction in Domestic Animals = Zuchthygiene·R HermesTb Hildebrandt
Mar 15, 2006·International Journal of Andrology·M Sa-ArdritA Pinyopummin
Nov 26, 2018·Reproduction in Domestic Animals = Zuchthygiene·Davide MonacoNikorn Thongtip
Jun 15, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·J-C LuN-Q Lu
Feb 9, 2018·Zoological Science·Sebastian J CharltonJulie Strand

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