Evaluation of Probiotic Potential and Safety Assessment of Lactobacillus pentosus MMP4 Isolated From Mare's Lactation

Probiotics and Antimicrobial Proteins
Jyoti ChoudharyShrivardhan Dheeman

Abstract

Lactic acid bacteria isolated from indigenous milk of different animals were investigated for their efficacy, safety, and probiotic potential. The most potential isolate MMP4 was screened from mare's milk, which was further identified as Lactobacillus pentosus by using 16S rRNA gene sequencing and phylogeny. The probiotic potential of strain MMP4 was assessed by its ability to survive under acidic environment and in presence of bile salts along with the ability to inhibit food-borne as well as clinical pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Salmonella typhi. The phenol tolerance with cogent hydrophobicity to different hydrocarbons was demonstrated. Bile salt hydrolase activity of L. pentosus MMP4 was confirmed by detecting the Bsh gene by using colony PCR. The presence of Mub, Map, and EF-Tu genes involved in adhesion conferred the behavior of passage and adherence to gastrointestinal tract. Scanning electron microscopy of intestinal autopsy from albino mice revealed the attachment of bacterial cells on the mucus-lined intestinal walls against pathogens and further proved in vivo adhesion ability. The presence of intrinsic antibiotic resistance and lack of DNase, ge...Continue Reading

References

Jun 1, 1995·Trends in Genetics : TIG·A Pospiech, B Neumann
Jan 5, 1999·Journal of Food Protection·W P CharterisJ K Collins
Feb 24, 1999·Critical Reviews in Food Science and Nutrition·A S NaiduR A Clemens
May 19, 2000·Microbes and Infection·R J Doyle
Feb 13, 2001·Journal of Biotechnology·M SaarelaT Mattila-Sandholm
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·N Cermakian, P Sassone-Corsi
Jan 5, 2002·Journal of Food Protection·W P CharterisJ K Collins
Nov 9, 2002·International Journal of Food Microbiology·R TemmermanJ Swings
Mar 1, 2006·International Journal of Food Microbiology·Maria G Vizoso PintoWilhelm H Holzapfel
Aug 26, 2006·Colloids and Surfaces. B, Biointerfaces·Jianhua PanHongxiao Tang
Aug 30, 2007·The Journal of General and Applied Microbiology·Kanchi Bhasker Praveen Kumar ReddySiddlingiaya Gurudutt Prapulla
Jun 10, 2008·International Journal of Food Microbiology·Julius Maina MatharaWilhelm H Holzapfel
Jan 11, 2011·International Journal of Food Microbiology·Heejae LeeWilhelm Holzapfel
Feb 22, 2012·Current Opinion in Critical Care·Lee E MorrowMark A Malesker
May 25, 2012·Nature
Nov 10, 2012·Izvestiia Akademii nauk. Seriia biologicheskaia·G M ZenovaD G Zviagintsev
Mar 29, 2014·Probiotics and Antimicrobial Proteins·Aabha Gupta, Santosh Kumar Tiwari
Aug 15, 2014·Probiotics and Antimicrobial Proteins·Ana Beatriz Jeronymo-CenevivaAna Lúcia B Penna
Mar 1, 2012·Probiotics and Antimicrobial Proteins·Tejinder Pal SinghSuman Kapila
Apr 20, 2016·Annals of Medicine·Arthur C OuwehandBuffy Stahl

❮ Previous
Next ❯

Citations

May 18, 2020·Probiotics and Antimicrobial Proteins·Hugo Calixto FonsecaRosane Freitas Schwan
Oct 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weiwei WangHuili Pang
May 11, 2021·Journal of Microbiological Methods·Jesús A Salas-TovarDavid R Sepulveda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.