Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR

Journal of Microbiological Methods
Sami SlimaniChristophe Ginevra

Abstract

A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria.

References

Aug 30, 2001·Applied and Environmental Microbiology·N WellinghausenR Marre
Apr 7, 2006·Applied and Environmental Microbiology·Philippe JolySophie Jarraud
Dec 30, 2006·Applied and Environmental Microbiology·Diaraf Farba YaradouSophie Jarraud
Aug 28, 2007·International Journal of Hygiene and Environmental Health·Florent MorioAlain Reynaud
Oct 14, 2008·Applied and Environmental Microbiology·Séverine AllegraSerge Riffard
Jul 16, 2010·Microbial Ecology·Mariana FittipaldiJordi Morató
Feb 19, 2011·Journal of Microbiological Methods·M Adela YáñezVicente Catalán

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Citations

Sep 26, 2013·The Journal of Hospital Infection·P CassierS Jarraud
May 6, 2015·International Journal of Hygiene and Environmental Health·Nicole ZachariasChristiane Schreiber
Oct 15, 2013·Journal of Applied Microbiology·P ElizaquívelG Sánchez
Jan 8, 2015·Frontiers in Microbiology·Peter RobertsonRafael A Garduño
Sep 23, 2014·Molecular and Cellular Probes·Savina DitommasoCarla M Zotti
May 3, 2016·Diagnostic Microbiology and Infectious Disease·Maria ScaturroMaria Luisa Ricci
Apr 28, 2017·International Journal of Environmental Research and Public Health·Sara BonettaElisabetta Carraro
Oct 5, 2018·Journal of Water and Health·Claudia Patricia AcostaJordi Morató
Oct 28, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Rehan DeshmukhUtpal Roy
Mar 21, 2020·Applied Microbiology and Biotechnology·Wenfang LinXin Yu
Jan 15, 2015·Environmental Science & Technology·Shenghua ZhangXin Yu
Mar 15, 2014·Environmental Science & Technology·Tzu-Hsin ChiaoLutgarde Raskin

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