Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs

Journal of Ethnopharmacology
Hao TangShengxiang Jiang

Abstract

Chi-Zhi-Huang decoction (PGR) is one of the traditional Chinese medicine (TCM) preparations with unique effect on withdrawing jaundice and has been used to treat icteric patients in China for many years. In this research, we aim at to evaluate the potential activity of PGR in restoring hepatic drug metabolism in a damaged liver. A cocktail approach with caffeine (10mg/kg), dapsone (10mg/kg) and chlorzoxazone (20mg/kg) respectively as probe drug of cytochrome P450 (CYP) isoform of CYP 1A2, 3A4 and 2E1 was used to evaluate its possible effects on Phase I oxidative metabolism. Pretreated with three dosages of PGR water extract (0.75, 1.5 and 3g/kg, po) for 5 days, male Wistar rats (220-240 g) were intoxicated by phenylisothiocyanate (PITC, 100mg/kg, po) 24h before probes intravenous injection. The pharmacokinetics of the probes in the blood was determined simultaneously by HPLC, and their non-compartmental parameters were used to evaluate the metabolic difference among the groups. Moreover, the levels of liver enzymes (ALT, AST, ALP) and bilirubins were also measured for insight of liver function. The findings in this study suggest that PGR induces CYP 3A4, does not have much effect on CYP 2E1, and inhibits CYP 1A2 at high dosage....Continue Reading

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Citations

Aug 1, 2013·Indian Journal of Pharmaceutical Sciences·R A XuJ S Ma
Nov 5, 2014·BMC Complementary and Alternative Medicine·Haixing YangYonghua Wang
Jun 29, 2010·Research in Veterinary Science·Ling-Ling ZhangShan-Xiang Jiang
Nov 11, 2010·Journal of Veterinary Pharmacology and Therapeutics·L L ZhangS X Jiang
May 26, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Rachumallu RamakrishnaRabi Sankar Bhatta

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