Evaluation of pulsed-field gel electrophoresis profiles for identification of Salmonella serotypes.

Journal of Clinical Microbiology
Wen ZouJames J Chen

Abstract

Pulsed-field gel electrophoresis (PFGE) is a standard typing method for isolates from Salmonella outbreaks and epidemiological investigations. Eight hundred sixty-six Salmonella enterica isolates from eight serotypes, including Heidelberg (n = 323), Javiana (n = 200), Typhimurium (n = 163), Newport (n = 93), Enteritidis (n = 45), Dublin (n = 25), Pullorum (n = 9), and Choleraesuis (n = 8), were subjected to PFGE, and their profiles were analyzed by random forest classification and compared to conventional hierarchical cluster analysis to determine potential predictive relationships between PFGE banding patterns and particular serotypes. Cluster analysis displayed only the underlying similarities and relationships of the isolates from the eight serotypes. However, for serotype prediction of a nonserotyped Salmonella isolate from its PFGE pattern, random forest classification provided better accuracy than conventional cluster analysis. Discriminatory DNA band class markers were identified for distinguishing Salmonella serotype Heidelberg, Javiana, Typhimurium, and Newport isolates.

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Citations

Mar 13, 2012·Briefings in Bioinformatics·Wei-Jiun Lin, James J Chen
Apr 5, 2013·Journal of Clinical Microbiology·Matthew L RanieriMartin Wiedmann
Aug 14, 2013·PloS One·Hung-Chia ChenJames J Chen
Jul 25, 2015·International Journal of Food Microbiology·Chien-Shun ChiouYen-Yi Liu
May 1, 2016·Diagnostic Microbiology and Infectious Disease·Geehan SuleymanRobert J Tibbetts
Nov 16, 2013·Critical Reviews in Microbiology·Chunlei ShiAndrea Isabel Moreno Switt
Apr 26, 2016·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Geehan SuleymanGeorge Alangaden
Jul 29, 2016·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Xi LuoJiu-Jin Xiao
Jan 10, 2018·Frontiers in Microbiology·Rafaela G FerrariCarlos A Conte-Junior
Jun 19, 2015·Journal of Clinical Microbiology·R G Amachawadi, T G Nagaraja
Oct 12, 2013·Journal of Food Protection·Robert R MikschMansour Samadpour
Jul 30, 2019·Frontiers in Microbiology·Silin TangMartin Wiedmann
Feb 17, 2018·Microbiology Spectrum·Deepak Kumar, Siddhartha Thakur

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