PMID: 9646005Jul 1, 1998Paper

Evaluation of Puumala virus IgG and IgM enzyme immunoassays based on recombinant baculovirus-expressed nucleocapsid protein for early nephropathia epidemica diagnosis

Clinical and Diagnostic Virology
H Kallio-KokkoA Vaheri

Abstract

Puumala virus (PUU), a member of Hantavirus genus, is the causative agent of nephropathia epidemica (NE), a milder form of hemorrhagic fever with renal syndrome (HFRS). Rapid diagnosis is essential for clinical management of NE. To evaluate the usefulness of recombinant protein-based IgM (direct- and mu-capture) and IgG (direct-and antigen (Ag)-capture) enzyme immunoassays (EIA) in early diagnosis of NE in comparison to IgG immunofluorescence assay (IF), and to find out the time limit for PUU-specific antibody seroconversion. The specific IgM and IgG antibody responses in serum were analyzed in 109 patients (235 serial sera) and 114 patients (233 serial sera), respectively. The serum panel used was selected from a larger material according to the availability of information concerning the date after onset of symptoms, the panel also containing NE patients who had been IgG-IF negative in their first (early) samples to find out the possible differences between sensitivities of the EIAs and IF. All NE patients tested became IgM-positive at the latest on the 6th (mu-capture EIA) or 7th (direct-IgM EIA) day after onset of symptoms. Out of a panel of very early NE-patient sera (n = 38) that could not be detected by IgG-IF, 66% were a...Continue Reading

References

Oct 1, 1992·Journal of Medical Virology·T Avsic-ZupancJ W LeDuc
Nov 30, 1991·Lancet·K HedmanM Brummer-Korvenkontio
Feb 1, 1980·The Journal of Infectious Diseases·M Brummer-KorvenkontioJ Lähdevirta
Oct 1, 1996·Journal of Virological Methods·A IvanovA Lundkvist
Nov 1, 1996·The Journal of General Virology·A PlyusninA Vaheri
Sep 23, 1997·Lancet·A LundkvistA Plyusnin

❮ Previous
Next ❯

Citations

Oct 7, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Jirí DusekJan Janda
Mar 22, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E SeitsonenV Pettilä
May 25, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jonas SchmidtRainer Ulrich
Mar 31, 2004·Microbes and Infection·Masanori TerajimaFrancis A Ennis
Sep 2, 1999·Journal of Virological Methods·K B Sjölander, A Lundkvist
Nov 16, 2001·Microbes and Infection·D H KrügerA Lundkvist A
Sep 21, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·P KorakaJ Groen
Mar 20, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J SchubertB Weissbrich
Oct 12, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H HujakkaA Närvänen
Sep 29, 2009·Epidemiology and Infection·K VapalahtiO Vapalahti
May 25, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Els KeyaertsJan Clement
Jan 7, 2003·Viral Immunology·Svetlana F Khaiboullina, Stephen C St Jeor
Jan 27, 2005·Viral Immunology·Piet MaesMarc Van Ranst
May 29, 2001·Journal of Clinical Microbiology·H HujakkaO Vapalahti
Oct 9, 2003·Journal of Clinical Microbiology·Boris KlempaDetlev H Krüger
Oct 22, 2004·Emerging Infectious Diseases·Franz Maximilian RascheLutz von Müller
Oct 1, 2009·Emerging Infectious Diseases·Maria Söderlund-VenermoKlaus Hedman
Oct 3, 2003·The Lancet Infectious Diseases·Olli VapalahtiAntti Vaheri
Mar 4, 2009·Expert Review of Anti-infective Therapy·Paul HeymanTatjana Avsic-Zupanc
May 30, 2009·Journal of Virological Methods·Anne J JääskeläinenHeli Piiparinen
Jul 1, 2008·Journal of Virological Methods·Mariana G BegoStephen St Jeor
Sep 11, 2007·Journal of Virological Methods·Sang Myun Park, Jongsun Kim
Oct 1, 2004·Acta Paediatrica·J van der Werff ten BoschD Piérard
May 9, 2008·Reviews in Medical Virology·Antti VaheriAlexander Plyusnin
Jan 25, 2005·Journal of Virological Methods·Mohamed AitichouM Sofi Ibrahim
Apr 23, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T Avšič-ŽupancM Korva
Dec 12, 2012·Diagnostic Microbiology and Infectious Disease·Sari M ViitalaAle Närvänen
Apr 10, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Astrid MuyldermansVeroniek Saegeman
May 15, 2015·Journal of Clinical Microbiology·Satu HepojokiOlli Vapalahti
Dec 18, 2013·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·O EnglerM Mutsch
Dec 7, 2019·Acta Radiologica·Olivier Lebecque, Michaël Dupont
May 9, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J ClementM Van Ranst
Jun 2, 2000·Journal of Clinical Microbiology·K Brus SjölanderA Lundkvist

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.