Evaluation of rapid KPC carbapenemase detection method based on MALDI-TOF VITEK MS spectra analysis

Journal of Medical Microbiology
Anna Rita CentonzeAnnarita Mazzariol

Abstract

Clinical microbiology laboratories in hospital settings need to be able to identify patients who carry carbapenemase-producing bacterial strains quickly in order to contain their spread and initiate proper pharmacological therapy. The aim of this study was to confirm the correlation between KPC production and a characteristic mass spectrometry (MS) peak (11 109 Da±8) to validate the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS as a rapid screening tool. With this aim, 176 selected clinical samples that were KPC-producing and 260 control samples that were carbapenem-susceptible or carbapenem-resistant through other resistance mechanisms, or were producing hydrolytic enzymes other than KPC, were analysed. The presence of the 11 109 Da peak in the spectra of 99.4 % (175/176) of the KPC-producing strains compared to the controls, which all lacked the peak, confirmed a strong correlation between KPC production and the presence of the 11 109 Da peak in the MALDI-TOF MS spectrum. The high sensitivity (98.7 %) and specificity (100 %) of the peak searching in the MALDI-TOF MS spectra mean that 11 109 Da peak searching is a suitable screening tool in KPC-endemic regions.

References

Jul 1, 1996·Applied and Environmental Microbiology·A GötzK Smalla
Jun 7, 2005·Journal of Microbiological Methods·Alessandra CarattoliE John Threlfall
Nov 13, 2008·The Journal of Antimicrobial Chemotherapy·Aurora García-FernándezAlessandra Carattoli
Jan 15, 2010·The Journal of Antimicrobial Chemotherapy·Caroline DallenneGuillaume Arlet
Sep 10, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P R Murray
Aug 31, 2012·Emerging Infectious Diseases·Patrice NordmannLaurent Dortet
Nov 30, 2013·Letters in Applied Microbiology·Y Hoyos-MallecotJ M Navarro-Marí
Nov 16, 2014·Journal of Clinical Microbiology·Sally R Partridge
Aug 24, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A R CentonzeA Mazzariol

❮ Previous
Next ❯

Citations

Jun 30, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ioannis K Neonakis, Demetrios A Spandidos
Mar 11, 2021·International Journal of Systematic and Evolutionary Microbiology·Xiaojun ZhouLina Niu
Feb 18, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E AntinoriA Mazzariol
Nov 30, 2021·Journal of Microbiological Methods·Yun HuangCongrong Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.