Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting.

BMC Infectious Diseases
Maria Cristina Carvalho Espírito-SantoRonaldo Cesar Borges Gryschek

Abstract

Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato-Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE. A cross-sectional study conducted from April to December 2011 using a probabilistic sampling that collected 572 fecal and serum samples. The laboratory diagnostic techniques used were: KK, HH and qPCR (fe...Continue Reading

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Citations

Dec 23, 2016·PLoS Neglected Tropical Diseases·Kenneth R HawkinsTala de Los Santos
Jan 4, 2018·Parasitology·Maria Teresinha Gomes CasavechiaJorge Juarez Vieira Teixeira
Sep 12, 2019·PLoS Neglected Tropical Diseases·Hélène GueganXavier Iriart
Mar 31, 2016·Infectious Diseases of Poverty·Ping HeZhi-Yue Lv
Jun 24, 2020·Infectious Diseases of Poverty·Antje FussAndreas Mueller
Oct 3, 2018·Tropical Medicine and Infectious Disease·Kosala G WeerakoonDonald P McManus
Sep 3, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hong-Mei LiXiao-Nong Zhou

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Datasets Mentioned

BETA
M61098

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
Excel

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