Evaluation of recombinant antigen-based assays for diagnosis of bullous autoimmune diseases

Clinical and Diagnostic Laboratory Immunology
G D'AgostoP Cordiali Fei

Abstract

The diagnosis of autoimmune bullous diseases is based on clinical observation and on the presence of autoantibodies directed to molecules involved in the adhesion systems of the skin. Immunofluorescence assays are the currently accepted method for detection of autoantibodies; such assays depend greatly on the skill of operators and are difficult to standardize. Recombinant desmoglein-1 (Dsg1), Dsg3, and BP180 peptides, the main autoantigens in pemphigus or bullous pemphigoid, have been used to develop new quantitative enzyme immunoassays (EIA) for the detection of specific antibodies. The present study was undertaken to evaluate the sensitivity and specificity of these immunoassays and to determine the correlation between the results and the clinical aspects of diseases. Serum samples from patients with pemphigus vulgaris, pemphigus foliaceus, bullous pemphigoid, or mucous membrane pemphigoid, from healthy individuals, and from patients with unrelated autoimmune conditions were tested. Anti-desmoglein reactivity was detected in all the patients with pemphigus and in none of the controls. Patients with the more benign form of cutaneous disease had anti-Dsg1 antibodies, while patients with deeper cutaneous lesions or with mucosal...Continue Reading

References

May 1, 1989·The Journal of Clinical Investigation·J R Stanley
Jun 1, 1995·The Journal of Investigative Dermatology·M AmagaiT Nishikawa
Feb 18, 1999·The Journal of Clinical Investigation·M G MahoneyJ R Stanley
May 21, 1999·Archives of Dermatological Research·D Zillikens, G J Giudice
Jun 24, 1999·Journal of Dermatological Science·D Zillikens
Nov 4, 2000·Expert Opinion on Investigational Drugs·N Braun, T Bosch
Oct 18, 2001·Clinics in Dermatology·A M Powell, M Black
Nov 14, 2001·Clinics in Dermatology·L S Chan
Nov 17, 2001·Journal of the American Academy of Dermatology·D F Mutasim, B B Adams
Apr 5, 2003·Archives of Dermatological Research·Takashi Hashimoto

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Citations

Jun 29, 2015·Autoimmunity Reviews·Mayson B MustafaCassian Sitaru
Sep 26, 2019·Frontiers in Immunology·Sandra SaschenbreckerWolfgang Schlumberger
Jul 5, 2017·Archives of Dermatological Research·Lajevardi VahideSaljoughi Nazi

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