Evaluation of recombinant Chinese avian hepatitis E virus (CaHEV) ORF2 and ORF3 proteins for protection of chickens against CaHEV infection

Vaccine
Shahid Faraz SyedQin Zhao

Abstract

Avian hepatitis E virus (HEV) is the etiologic agent of big liver and spleen disease in chickens. In 2010, the Chinese avian HEV (CaHEV) strain was isolated from chickens and demonstrated to cause the decreased egg production in layer hens. No avian HEV commercial vaccine has yet been developed to prevent virus infection in China. In this study, recombinant CaHEV truncated ORF2 and complete ORF3 proteins were evaluated separately for immunoprotection of chickens against CaHEV infection. First, truncated ORF2 and complete ORF3 proteins were expressed in Escherichia coli. Next, 48 specific-pathogen-free chickens were randomly divided into three groups. One group was immunized with truncated ORF2 protein, the second group was immunized with recombinant ORF3 protein, while the third group (control) was mock-immunized with PBS. After booster immunization, chickens in all three groups were challenged intravenously with CaHEV infectious stock and assessed for viremia, fecal virus shedding, seroconversion, and gross hepatic lesions. In the ORF2 protein-immunized group, no chickens showed evidence of avian HEV infection. In the ORF3 protein-immunized group, nine chickens exhibited viremia and seven had fecal virus shedding. In the contr...Continue Reading

Citations

Dec 20, 2017·Frontiers in Microbiology·Yuchen NanEn-Min Zhou
Aug 10, 2019·Frontiers in Microbiology·Peng SunQin Zhao
Jun 8, 2021·World Journal of Gastroenterology : WJG·Yong-Lin Yang, Yu-Chen Nan
Jul 18, 2021·Cellular Microbiology·Mirco Glitscher, Eberhard Hildt
Oct 12, 2021·Applied Microbiology and Biotechnology·Beibei ZhangEn-Min Zhou

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