Abstract
Vaccines based on gamete specific proteins have been proposed for fertility inhibition. In the present study, immunogenicity and contraceptive potential of E. coli-expressed recombinant fusion protein TT-KK-ZP3-GGG-Iz, comprising promiscuous T cell epitope of tetanus toxoid (TT) followed by dilysin linker (KK), a fragment of dog zona pellucida glycoprotein-3 (ZP3), triglycine spacer (GGG) and a fragment of dog Izumo (Iz) without any affinity tag has been evaluated in female FvB/J mice. In addition, recombinant TT-KK-ZP3 and Izumo linked to the promiscuous T cell epitope of bovine RNase (bRNase-KK-Iz) and their physical mixture were also used. SDS-PAGE and immunoblot studies revealed ∼32kDa band corresponding to TT-KK-ZP3-GGG-Iz, ∼22kDa band of TT-KK-ZP3 and ∼11kDa band of bRNase-KK-Iz. Groups of mice immunized with the above recombinant proteins led to the generation of high antibody titres against the respective proteins. Immunization with recombinant TT-KK-ZP3-GGG-Iz generated higher antibody titre as compared to mice immunized with physical mixture of TT-KK-ZP3 and bRNase-KK-Iz. Antibodies against TT-KK-ZP3-GGG-Iz and TT-KK-ZP3 recognized mouse and dog ZP and those against TT-KK-ZP3-GGG-Iz and bRNase-KK-Iz recognized mouse a...Continue Reading
References
Jan 1, 1975·Journal of Reproduction and Fertility·M H Sellens, E J Jenkinson
Mar 1, 1990·European Journal of Immunology·P C HoC M Rzepczyk
Aug 15, 1970·Nature·U K Laemmli
Sep 1, 1994·Journal of Reproduction and Fertility·H BagavantS K Gupta
Jan 1, 1997·Reproduction, Fertility, and Development·K Stöhr, F X Meslin
Oct 6, 1999·Current Opinion in Biotechnology·F Baneyx
Feb 13, 2001·Oral Microbiology and Immunology·Y OishiT Nisizawa
Jan 16, 2002·Current Opinion in Immunology·Ana-Maria Lennon-DuménilCécile Lagaudrière-Gesbert
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·N SrivastavaS K Gupta
Mar 16, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Christopher M HardyMalcolm A Lawson
Mar 11, 2005·Nature·Naokazu InoueMasaru Okabe
Apr 2, 2005·Protein Expression and Purification·Jordan J LichtySong Tan
Feb 10, 2007·Molecular Reproduction and Development·Ding LiChen Xu
Sep 4, 2007·The Veterinary Record·J F Reece
Sep 11, 2007·Vaccine·John WalkerDavid C Jackson
Feb 28, 2009·Biology of Reproduction·Pankaj BansalSatish K Gupta
Nov 9, 2010·Vaccine·Christi A Yoder, Lowell A Miller
Mar 8, 2011·Journal of Reproductive Immunology·Satish K GuptaV A Srinivasan
Nov 6, 2012·Fertility and Sterility·Pankaj SumanSatish Kumar Gupta
Dec 18, 2012·Molecular Biotechnology·Neha GuptaSatish Kumar Gupta
Mar 1, 2013·American Journal of Reproductive Immunology : AJRI·Neha GuptaSatish K Gupta
Jul 23, 2013·Vaccine·Fernando Vargas-PinoDennis Slate
Citations
Feb 10, 2016·Journal of Reproductive Immunology·Ananta Prasad ArukhaSatish Kumar Gupta
Feb 6, 2016·Reproductive Biomedicine Online·Mei HanJie Zhao
Dec 3, 2014·Vaccine·Abhinav ShresthaSatish Kumar Gupta
Dec 29, 2015·Equine Veterinary Journal·C J JoonèM L Schulman
Apr 12, 2014·Protein Science : a Publication of the Protein Society·Rajesh K Naz
May 13, 2018·Reproductive Biology and Endocrinology : RB&E·Ying WangFuchun Zhang
Oct 2, 2019·International Journal of Molecular Sciences·Takako SaitoNaokazu Inoue