Evaluation of replicative capacity and genetic stability of West Nile virus replicons using highly efficient packaging cell lines

Virology
Rafik FayzulinPeter W Mason

Abstract

A stable cell system for high-efficiency packaging of West Nile virus (WNV) subgenomic replicons into virus-like particles (VLPs) was developed. VLPs could be propagated on these packaging cells and produced infectious foci similar to foci produced by WNV. Focus size correlated with the replicative capacity of WNV replicons, indicating that genome copy number, rather than amount of trans-complementing structural proteins, was rate-limiting in packaging of VLPs. Comparison of VLP production from replicon genomes encoding partial or complete C genes indicated that portions of C downstream of the cyclization sequence could improve genome replication or that cis expression of C could enhance packaging. Interestingly, a rapid loss of replicon-encoded reporter gene activity was detected within two serial passages of reporter gene-containing VLPs. The loss of reporter activity correlated with gene deletion and better VLP growth, indicating a powerful selection pressure for WNV genomes lacking reporter genes.

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Citations

Aug 24, 2007·Journal of Virology·Alexandr V ShustovIlya Frolov
Jun 20, 2008·Journal of Virology·Jason R WilsonFrank Scholle
Apr 17, 2009·Journal of Virology·Guruprasad R MedigeshiJay A Nelson
Oct 6, 2012·The Veterinary Journal·Akihiko Maeda, Junko Maeda
Jun 24, 2008·Antiviral Research·Hongping DongPei-Yong Shi
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Jul 23, 2016·Journal of Virological Methods·Ming-Yue XuBo Zhang
Mar 6, 2013·Anais Da Academia Brasileira De Ciências·Sabrina R A QueirozLaura H V G Gil
Feb 23, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer L UhrlaubJanko Nikolich-Zugich

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