Evaluation of RIDASCREEN® and RIDA®QUICK Helicobacter kits for Helicobacter pylori detection in stools.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Lucie BénéjatPhilippe Lehours

Abstract

The diagnosis of Helicobacter pylori infection can be made by using noninvasive tests. The detection of bacterial antigens in stool samples is a technique proposed by some suppliers. The objective of this study was to evaluate retrospectively the performances of the commercially available RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter (R-Biopharm) kits in detecting H. pylori antigens in stool samples. A collection of 132 stools was used in this study: 94 stools obtained from H. pylori-negative patients and 38 stools from H. pylori-positive patients. The performances (sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)) were evaluated for the RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter kits in comparison with real-time PCR results performed on gastric biopsies as well as culture. Discordant results, with respect to H. pylori status, were checked on the same day as the test by repeating the procedure. All of the readings concerning the RIDA®QUICK Helicobacter tests were concordant between 3 users, i.e., 94/94 negative tests and 34/38 positive tests. RIDASCREEN® Helicobacter tests were negative for all 94 H. pylori-negative samples and positive for 35/38 positive stools. Readin...Continue Reading

References

Jun 20, 2006·The American Journal of Gastroenterology·Javier P GisbertVíctor Abraira
Apr 13, 2007·Clinical Microbiology Reviews·Francis Mégraud, Philippe Lehours
Apr 12, 2011·Journal of Pediatric Gastroenterology and Nutrition·Yelda A LealJavier Torres
Jun 28, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A DucournauF Mégraud
Nov 2, 2016·Gut·P MalfertheinerUNKNOWN European Helicobacter and Microbiota Study Group and Consensus panel
Mar 16, 2018·The Cochrane Database of Systematic Reviews·Lawrence Mj BestKurinchi Selvan Gurusamy

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Methods Mentioned

BETA
biopsies
ELISAs
PCR
ELISA

Software Mentioned

RIDASCREEN®

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