PMID: 8582143Aug 1, 1995Paper

Evaluation of S1 chromogenic cephalosporin beta-lactamase disk assay tested against gram-positive anaerobes, coagulase-negative staphylococci, Prevotella spp. and Enterococcus spp

Diagnostic Microbiology and Infectious Disease
S A MarshallR N Jones

Abstract

The efficacy of three rapid colorimetric disk assays to detect beta-lactamase production in 60 clinical isolates was evaluated. Two chromogenic cephalosporin substrates (S1 and nitrocefin) and an acidimetric test were in complete agreement when tested against Enterococcus spp. (20 strains, not Enterococcus faecalis), Prevotella spp. (10 strains) and Gram-positive anaerobic cocci (10 strains). However, the acidimetric test produced documented false-negative results in detecting the beta-lactamases from coagulase-negative staphylococci (two of 20 strains tested). The time required to produce a positive result for the discordant Staphylococcus epidermidis isolate favored S1 compared with nitrocefin. These studies indicate that the acidimetric test was less sensitive than the chromogenic cephalosporin substrates and that nitrocefin and S1 could be used to screen for beta-lactamase production in these tested species.

References

Aug 21, 1992·Science·H C Neu
Nov 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A C PeterssonH Miörner
Feb 1, 1995·Diagnostic Microbiology and Infectious Disease·J F Tomayko, B E Murray

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.