PMID: 9431964Feb 12, 1998Paper

Evaluation of screening and commercial methods for detection of methicillin resistance in coagulase-negative staphylococci

Journal of Clinical Microbiology
Z HussainB Nancekivell

Abstract

The National Committee for Clinical Laboratory Standards recommends 48 h of incubation by the oxacillin salt agar screen (OSAS) method for the detection of methicillin-resistant coagulase-negative staphylococci (CoNS). An earlier identification of methicillin resistance is desirable. The time to detection of the mecA gene by PCR was compared with the times to detection by OSAS, by the oxacillin disk diffusion (ODD) method, and with MicroScan Gram Positive Combo type 6 panels (MicroScan Inc. Sacramento, Calif.) and Vitek GPS-SA cards (bioMérieux Vitek Inc., Hazelwood, Mo.). The combination of the Vitek card and the ODD method detected 92 of 99 methicillin-resistant strains of CoNS at 24 h; however, 6 mecA-positive strains were phenotypically methicillin susceptible. We conclude that most methicillin-resistant CoNS can be detected and the results can be reported after overnight incubation by a combination of methods.

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Citations

Jul 25, 2006·Memórias do Instituto Oswaldo Cruz·Juliana CaierãoPedro Alves d'Azevedo
Jul 8, 2011·Clinical Microbiology Reviews·Trevor Winstanley, Patrice Courvalin
Jan 19, 2008·Journal of Medical Microbiology·Pedro A d'AzevedoAntonio C Pignatari
Aug 2, 2006·Biological & Pharmaceutical Bulletin·María Cristina Gaudioso de AlloriMarta Elena Cecilia de Castillo
Aug 30, 2001·Journal of Clinical Microbiology·W J MasonM S Smeltzer
Aug 31, 2002·Journal of Clinical Microbiology·Matthias A HorstkotteDietrich Mack

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