Evaluation of Serological SARS-CoV-2 Lateral Flow Assays for Rapid Point-of-Care Testing.

Journal of Clinical Microbiology
Steven E ConklinOliver Laeyendecker

Abstract

Rapid point-of-care tests (POCTs) for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies vary in performance. A critical need exists to perform head-to-head comparisons of these assays. The performances of 15 different lateral flow POCTs for the detection of SARS-CoV-2-specific antibodies were compared on a well-characterized set of 100 samples. Of these, 40 samples from known SARS-CoV-2-infected, convalescent individuals (collected an average of 45 days after symptom onset) were used to assess sensitivity. Sixty samples from the prepandemic era (negative control) that were known to represent infections with other respiratory viruses (rhinoviruses A, B, and C and/or coronavirus 229E, HKU1, and NL63 OC43) were used to assess specificity. The timing of seroconversion was assessed using five lateral flow assays (LFAs) and a panel of 272 longitudinal samples from 47 patients for whom the time since symptom onset was known. Among the assays that were evaluated, the sensitivity and specificity for any reactive band ranged from 55% to 97% and from 78% to 100%, respectively. Assessing the performance of the IgM and the IgG bands alone, sensitivity and specificity ranged from 0% to 88% and 80% ...Continue Reading

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Citations

May 2, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mamdouh SibaiBenjamin A Pinsky
May 21, 2021·The American Journal of Emergency Medicine·Oliver LaeyendeckerUNKNOWN Johns Hopkins COVID-19 EM Investigators
Jun 3, 2021·Nanomaterials·Elena ZavyalovaVladimir Kukushkin
May 30, 2021·Biosensors & Bioelectronics·Luyang WangLingxin Chen
Apr 28, 2021·Journal of Clinical Microbiology·Owen R BakerOliver Laeyendecker
Jul 31, 2021·Biomicrofluidics·Hao YuanChien-Fu Chen
Aug 24, 2021·Frontiers in Bioengineering and Biotechnology·Guozhen Liu
Oct 21, 2021·ACS Nano·André Lopes FerreiraCesar de la Fuente-Nunez
Oct 26, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Christopher D HeaneyAaron A R Tobian

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