Evaluation of serum and ocular levels of membrane attack complex and C-reactive protein in CFH-genotyped human donors.

Eye
Kathleen R ChircoRobert F Mullins

Abstract

There is a considerable body of evidence demonstrating a link between the membrane attack complex (MAC) and age-related macular degeneration (AMD), and between C-reactive protein (CRP) and AMD. Both the MAC and the monomeric form of CRP (mCRP) accumulate within the choriocapillaris in AMD. However, the precise contribution of these species to AMD pathophysiology has not been fully elucidated. We sought to directly assess CRP and MAC levels between human serum and ocular tissues from the same CFH Y402H genotyped donors using ELISA of serum and RPE/choroid proteins. The Y402H polymorphism was associated with significantly increased MAC in RPE/choroid samples, but not in the serum, in a previously unstudied cohort. While MAC levels in the choroid were independent of circulating levels, choroidal CRP was correlated to serum levels. These data provide further evidence for local activation of complement within the choriocapillaris in AMD.

References

Mar 12, 2005·Science·Robert J KleinJosephine Hoh
Nov 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·P T JohnsonL V Johnson
Jun 3, 2011·The British Journal of Ophthalmology·Imran A BhuttoGerard A Lutty
Dec 9, 2014·Progress in Retinal and Eye Research·S Scott WhitmoreRobert F Mullins
Jul 5, 2016·The Journal of Pathology·Kathleen R ChircoRobert F Mullins

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Citations

Nov 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Andrew P VoigtRobert F Mullins
Dec 30, 2020·International Journal of Molecular Sciences·Emilie Grarup JensenThomas J Corydon

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Methods Mentioned

BETA
ELISA

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