Evaluation of serum protein separation by capillary electrophoresis: prospective analysis of 1000 specimens

Journal of Chromatography. B, Biomedical Applications
M A JenkinsM D Guerin

Abstract

To critically assess the method of capillary electrophoresis (CE) we examined 1000 prospective serum samples submitted for protein electrophoresis by both high-resolution agarose gel electrophoresis (HRAGE) and CE. CE was performed using a 72 cm (50 cm to detector) x 50 microns I.D. fused-silica capillary with detection of absorbance at 200 nm. The 1000 samples examined contained 362 monoclonal paraproteins with concentrations ranging from 1 to 71 g/l. We evaluated the individual paraprotein correlations, the overall correlation between the two methods being 0.96. We found that HRAGE gave slightly higher values for the monoclonal bands than CE and the difference was statistically significant. We conclude that CE is a viable alternative to HRAGE for the determination of protein dyscrasias in a routine clinical laboratory.

References

Sep 7, 1990·Journal of Chromatography·M D ZhuT Wehr
Oct 21, 1983·Science·J W Jorgenson, K D Lukacs

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Citations

May 19, 2001·Electrophoresis·C J StewartD Perrett
Feb 12, 1998·Electrophoresis·C L ColyerD J Harrison
Feb 12, 1998·Electrophoresis·M A Friedberg, Z K Shihabi
Feb 10, 1998·Electrophoresis·V Dolnik
Feb 10, 1998·Electrophoresis·F von Heeren, W Thormann
May 17, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maoyong SongGuoqing Shi
Mar 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·John R PetersenDeborah A Payne
Oct 8, 1999·Journal of Biochemical and Biophysical Methods·M A Jenkins, S Ratnaike
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·M A Jenkins, M D Guerin
May 10, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Michele MussapMario Plebani
Jan 8, 2014·Journal of Clinical Laboratory Analysis·Hongwei SongHui Cong
May 24, 2006·Electrophoresis·Rukhsana JabeenJohn Petersen
Oct 28, 2003·Clinical Chemistry·Cécile Gay-BellileThierry Le Bricon
Feb 26, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·David F Keren, Lee Schroeder
Jan 19, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M A Jenkins
Oct 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·R LehmannH M Liebich
Oct 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·K Kubo
Jun 28, 1996·Journal of Chromatography. B, Biomedical Applications·M A Jenkins, M D Guerin
Jul 21, 2000·Annals of Clinical Biochemistry·M A JenkinsJ Des Parkin
Jun 17, 1999·Annals of Clinical Biochemistry·D Perrett

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