Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA

Molecular Biology Reports
A RosnerH Aviv

Abstract

A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids or S. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared.

References

Jul 14, 1977·Nature·D A HopwoodS N Cohen
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D H Hamer, C A Thomas
Dec 30, 1975·Molecular & General Genetics : MGG·M SoS Falkow
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S N CohenL Hsu
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K TimmisS N Cohen
Oct 27, 1972·Biochimica Et Biophysica Acta·J Retèl, R J Planta

Related Concepts

Alkaline Phosphatase
Seromycin
Genes, Spliced
Alkalescens-Dispar Group
Study, Methodological
Episomes
Streptomyces griseus
Tetrabid

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