Abstract
The aim of the present work is to confirm the presence of MDF (myocardial depressant factor), which has long been postulated to be one of the cardiotoxic substances in the shock state. Twenty male mongrel dogs were divided into two groups, a hemorrhagic shock group (n = 10) and an endotoxic shock group (n = 10). Blood samples were obtained from each animal at 1, 2, 4, and 7 h after hypotensive events occurred. Inotropic properties of the plasma samples were evaluated by the isometric contraction of a cat papillary muscle preparation, and chromatographic analysis was performed on the peptides in the plasma. Developed tension of the muscle was increased significantly by changing the bathing medium from Krebs-Henseleit solution to plasma obtained 1 to 4 h after the onset of hemorrhagic and endotoxin induced hypotension. The positive inotropic change was associated with a significant increase in plasma epinephrine concentration. None of these plasma samples possessed a negative inotropic effect (i.e., the property of MDF activity). The elution profile by gel column chromatography of samples obtained from shocked animals was almost identical to that recognized as MDF. However, the presence of MDF was not confirmed by column paramete...Continue Reading
References
Jan 15, 1979·Experientia·M J Galvin, A M Lefer
Jul 1, 1979·Microvascular Research·R JusticeH B Hechtman
Mar 28, 1977·Biochimica Et Biophysica Acta·L J GreeneA M Lefer
Oct 1, 1985·The Journal of Clinical Investigation·J E ParrilloW Schuette
Sep 1, 1966·The American Journal of Physiology·A M LeferE D Brand
Sep 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·L B HinshawC A Guenter
Feb 1, 1973·Circulation Research·A M Lefer
Nov 1, 1972·The American Journal of Physiology·A M Lefer, Y Barenholz
Apr 1, 1972·The American Journal of Physiology·L B HinshawC A Guenter
Jan 1, 1972·Cardiology·J S ForresterC W Urschel
Mar 1, 1973·The Journal of Trauma·S L WangensteenJ R Starling
Dec 1, 1973·The Journal of Surgical Research·L B HinshawL J Greenfield
Feb 1, 1974·The American Journal of Physiology·L B HinshawL J Greenfield
Feb 1, 1972·The American Journal of Physiology·W W FergusonA M Lefer
Jan 1, 1970·Circulation Research·A M Lefer, J Martin
Jan 1, 1966·The Journal of Clinical Investigation·W W SpinkE Seljeskog
May 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·E D Brand, A M Lefer
Jun 13, 1980·Journal of Chromatography·G P JackmanH Skews