Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes

Progress in Biomaterials
Traian V ChirilaDamien G Harkin

Abstract

Sericin and fibroin are the two major proteins in the silk fibre produced by the domesticated silkworm, Bombyx mori. Fibroin has been extensively investigated as a biomaterial. We have previously shown that fibroin can function successfully as a substratum for growing cells of the eye. Sericin has been so far neglected as a biomaterial because of suspected allergenic activity. However, this misconception has now been dispelled, and sericin's biocompatibility is currently indisputable. Aiming at promoting sericin as a possible substratum for the growth of corneal cells in order to make tissue-engineered constructs for the restoration of the ocular surface, in this study we investigated the attachment and growth in vitro of human corneal limbal epithelial cells (HLECs) on sericin-based membranes. Sericin was isolated and regenerated from the silkworm cocoons by an aqueous procedure, manufactured into membranes, and characterized (mechanical properties, structural analysis, contact angles). Primary cell cultures from two donors were established in serum-supplemented media in the presence of murine feeder cells. Membranes made of sericin and fibroin-sericin blends were assessed in vitro as substrata for HLECs in a serum-free medium...Continue Reading

Citations

Oct 13, 2017·Biomedical Materials·Zhi ChenGordon G Wallace
Jan 13, 2016·Macromolecular Bioscience·Benjamin P PartlowDavid L Kaplan
Jul 2, 2020·Biomaterials Science·Jugal Kishore SahooDavid L Kaplan
Mar 11, 2017·Scientific Reports·Chae Eun KimJaeWook Yang
Feb 22, 2017·Biotechnology and Applied Biochemistry·Traian V ChirilaChloé Papolla
Mar 8, 2016·Progress in Biomaterials·Masoud Dadras ChomachayiHamid Mirzadeh
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Methods Mentioned

BETA
electrophoresis

Software Mentioned

GraphPad Prism
OMNIC
FTÅ Drop Shape Analysis

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