Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis

Parasites & Vectors
Yuanbin ZhangWeiqing Pan

Abstract

Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits. The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-based ELISA kits to analyze the kinetics of antigen-specific antibodies during the course of infection in mice and rabbits. The early diagnostic validity of the candidate SjSP-216 was further evaluated in mice and rabbits infected with S. japonicum. Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs. In the mouse model, we detected a significant increase in antibodi...Continue Reading

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Citations

Jan 27, 2011·PLoS Neglected Tropical Diseases·Jing XuXiao-Nong Zhou
Jul 20, 2017·The American Journal of Tropical Medicine and Hygiene·Tilman LingscheidUNKNOWN TropNet Schistosomiasis Investigator Group
Aug 5, 2019·Parasitology Research·Adrian Miki C MacalandaShin-Ichiro Kawazu
May 20, 2021·Scientific Reports·Oyetunde Timothy OyeyemiRafaella Fortini Queiroz Grenfell

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Methods Mentioned

BETA
protein array
PCR
ELISA

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Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

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Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.