Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method

Applied Microbiology and Biotechnology
H AoshimaMotoki Kubo

Abstract

A simple and rapid method (slow-stirring method) for extracting environmental DNA (eDNA) from soils was constructed by physical mild stirring with chemical treatment. eDNA was extracted efficiently with minimal damage from various kinds of soil. The amount of eDNA and soil bacterial biomass showed a linear proportional relation [Y=(1.70x10(8))X, r2=0.96], indicating that bacterial biomass could be evaluated by quantifying levels of eDNA. Consequently, the average bacterial biomass in an agricultural field was calculated as 5.95x10(9) cells/g sample, approximately 10-100 times higher than that in non- and oil-polluted fields.

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Citations

Dec 18, 2008·Applied Biochemistry and Biotechnology·Kenzo KubotaMotoki Kubo
Mar 23, 2013·Applied Biochemistry and Biotechnology·Yuki FukuharaMotoki Kubo
Nov 10, 2013·The Journal of General and Applied Microbiology·Sachie HoriiMotoki Kubo
Sep 13, 2008·Biotechnology Advances·J Rajendhran, P Gunasekaran
Mar 11, 2016·Environmental Science & Technology·Yuan GePatricia Ann Holden
Jun 21, 2016·Journal of Environmental Radioactivity·Shin-Nosuke Hashida, Toshihiro Yoshihara
Sep 8, 2019·The ISME Journal·Xinda LuJosh D Neufeld
Apr 17, 2009·International Journal of Environmental Health Research·Stefan BorjessonPer-Eric Lindgren
Jan 7, 2011·Environmental Science & Technology·Yuan GePatricia A Holden

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