PMID: 6411774Sep 1, 1983Paper

Evaluation of some methods for the laboratory identification of Haemophilus influenzae

Journal of Clinical Pathology
G M Tebbutt

Abstract

Five tests--satellitism, synthesis of porphyrins, acid production from sucrose, beta-galactosidase activity (ONPG), and indole production--to differentiate between strains of Haemophilus influenzae and strains of V-dependent Haemophilus species were evaluated. Six per cent of strains of H influenzae were misidentified as H parainfluenzae by a test for satellitism using filter paper discs impregnated with X factor, V factor, or both, applied to Columbia Agar. None of seven nutrient agars tested grew Haemophilus species, and determined accurately the X factor requirement. Synthesis of porphyrins from delta-aminolaevulinic acid provided a reliable means of demonstrating that X factor was required. A test for the production of acid from sucrose discriminated successfully between strains of V-dependent Haemophilus species (positive) and H influenzae (negative). Most isolates were identified correctly by the ONPG test, but occasional V-dependent strains were negative and could be misidentified as H influenzae. The discriminative value of the indole test was unsatisfactorily low. The results of the tests are discussed in relation to the identification of H influenzae in the diagnostic laboratory.

References

Aug 1, 1978·Journal of Clinical Pathology·G M Tebbutt, D J Coleman
Mar 1, 1976·Journal of General Microbiology·M Kilian
Jan 1, 1983·Journal of Clinical Pathology·S Mehtar, S A Afshar
Feb 1, 1982·Journal of Clinical Microbiology·D F WelchJ M Matsen
Jan 1, 1963·Journal of Bacteriology·H L WOLIN
Jan 1, 1955·Acta Pathologica Et Microbiologica Scandinavica·V MØLLER
Feb 1, 1952·Journal of General Microbiology·P H CLARKE, S T COWAN

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Citations

Apr 1, 1984·Journal of Clinical Pathology·A Jones
Jul 1, 2012·Saudi Journal of Biological Sciences·N Abdel-RaoufI B M Ibraheem
Jun 1, 1990·The Journal of Applied Bacteriology·A de VicenteP Romero
Jan 10, 2017·Microbial Pathogenesis·Sana ShafiSuhaib A Bandh
Apr 1, 1984·Journal of Clinical Pathology·C RibeiroT Price
Jun 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·B BruunM Kilian

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