Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality

The Journal of Reproduction and Development
Kumiko TakedaShinya Watanabe

Abstract

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times over the course of a year, and the percentage of TUNEL-positive spermatozoa (sperm TUNEL index) was determined. Individual differences in semen were detected using the sperm TUNEL index in these bulls (P < 0.01). The sperm TUNEL index of cryopreserved semen obtained from test-mated Japanese Black (n = 30, including two bulls with a conception rate lower than 10%) and Holstein (n = 34) bulls were analyzed. The average sperm TUNEL index and conception rate resulting from artificial insemination (AI) were 4.7% and 55.7% for Japanese Black, and 4.9% and 39.5% for Holstein, respectively. A weak negative correlation between sperm TUNEL index and conception rate was observed in Holstein bulls (P < 0.05). Semen samples from six bulls with more than 10% sperm TUNEL index were studied, and these samples showed low sperm viability. However, semen res...Continue Reading

References

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Citations

Mar 23, 2017·The Journal of Reproduction and Development·Kumiko TakedaShinya Watanabe
Feb 9, 2018·Reproduction in Domestic Animals = Zuchthygiene·R SinghA Kumar
May 22, 2018·Animal : an International Journal of Animal Bioscience·S Fair, P Lonergan
Feb 23, 2018·Animal : an International Journal of Animal Bioscience·B R HarstineJ M DeJarnette
Jan 22, 2019·The Journal of Reproduction and Development·Kohei UmezuKentaro Tanemura
Jan 28, 2020·Reproduction in Domestic Animals = Zuchthygiene·Nunzia PaganoBianca Gasparrini
Dec 7, 2019·Journal of Animal Science and Biotechnology·MariaPortia B NagataKenichi Yamashita
Dec 21, 2017·Reproductive Medicine and Biology·Hiroshi HarayamaTaichi Noda
Sep 7, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Alexandra K TsarouchaConstantinos Simopoulos

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Methods Mentioned

BETA
electrophoresis
flow cytometry
fluorescence microscopy

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