Evaluation of staining methods of resin embedded cutaneous tissue sections of mycosis fungoides

The British Journal of Dermatology
A C ChuD M MacDonald

Abstract

Biopsies from lesional skin of patients with histologically confirmed mycosis fungoides were embedded in resin and semi-thin ( I micrometer) sections were submitted to a variety of staining procedures, including Toluidine blue, Richardson's Huber, Giemsa, Paragon and Tribasic stains. The stained sections were examined with the light microscope and the quality of cellular morphological detail afforded by the different stains was assessed. The Toluidine blue, Richardson's and Paragon stains were found to be technically easy to perform and gave the best representation of nuclear and cytoplasmic detail Of these, the authors favoured Richardson's stain. The technique of processing the tissue, and staining a mounting procedures are detailed.

References

Aug 1, 1966·American Journal of Clinical Pathology·J H MartinW M Nickey
Jan 1, 1969·Journal of Clinical Pathology·W N Eastham, W B Essex
Oct 13, 1956·Nature·A M GLAUERTG E ROGERS
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE

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Citations

Jun 1, 1981·The British Journal of Dermatology·S C Van der Putte, S P Tan-Ranoewisastro
Dec 1, 1982·The British Journal of Dermatology·D M MacDonald
Feb 1, 1982·Journal of Cutaneous Pathology·R J BarrJ E Buck
Oct 31, 2016·Journal of Cellular Physiology·Gerald J ShamiFilip Braet
Oct 1, 1985·Journal of Cutaneous Pathology·R Cerio, D M MacDonald

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